反式-4-羟基-L-脯氨酸发酵菌株的筛选

Screening of fermentation Strain for trans-4-hydroxy-L-proline

关键词:  反式-4-羟基-L-脯氨酸  发酵菌株  大肠杆菌  筛选

Key words:  trans-4-hydroxy-L-proline  fermentation strain  Escherichia coli  screening

摘要:

为选育出反式-4-羟基-L-脯氨酸发酵生产的优良菌株，以8株产反式-4-羟基-L-脯氨酸的大肠杆菌（Escherichia coli）为出发菌株，对其生长特性、发酵效率进行了比较分析，筛选出2株遗传稳定性好、生产效率高的菌株Hyp-Y1801、Hyp-Y1808，其发酵效价分别为50.4 g/L、47.5g/L，对实现反式-4-羟基-L-脯氨酸的工业化生产具有重要意义。

Abstract:

In order to select a good fermentation producing strain of trans-4-hydroxy-L-proline, comparative analysis were made in growth characteristics and production efficiency of 8 strains Escherichia coli producing trans-4-hydroxy-L-proline. Hyp-Y1801 strain and Hyp-Y1805 strain with high inheritance stability and high fermentation efficiency were screened. The fermentation potency of Hyp-Y1801 and Hyp-Y1808 were 50.4 g/L and 47.5 g/L respectively. The result had great significance for industrial production of trans-4-hydroxy-L-proline.