氟苯尼考注射液在鸡体内残留消除研究

Residue Deption Study of Florfenicol Injection in Chicken

关键词:  氟苯尼考  鸡  残留  休药期

Key words:  florfenicol  chicken  residue  withdrawal period

摘要:

［摘 要］为研究氟苯尼考注射液在鸡体内的残留消除规律。本实验采用35只约3kg左右的健康白羽鸡,随机分为2组,给药组30只,对照组5只。给药组用药量为20mg/kg/次,每隔48小时用药1次,连用2次,对照组不给任何抗菌药物,与给药组同环境饲养。在最后一次给药6h、24h(1天)、72h(3天)、120h(5天)、168h(7天)时采集肉、肝、肾、皮脂样本,经LC-MS/MS法测定组织中的氟苯尼考及其标示物氟苯尼考胺残留量,并利用WT1.4软件计算休药期。结果显示：氟苯尼考注射液在鸡肌肉、肝脏、肾脏及皮脂中的休药期分别是3.41d、2.06d、2.25d、1.47d。为保证兽药使用安全、消费者健康和食品安全,推荐氟苯尼考注射液在鸡体内的休药期为4d。

Abstract:

This experiment was designed to study the rule of diminishing of florfenicol in chickens. For that purpose, 35 healthy white broilers of about 3 kg in body weight were divided into 2 groups randomly, 30 in treatment group and 5 in control group. The dose of the drug for treatment group was 20 mg/kg/time, and the drug was injected 2 times consecutively, once in every 48 hours. The control group was not taken any antibacterial drugs and was kept in the same environment as the treatment group. Chicken meat, liver, kidney and sebum samples were collected 6h, 24h (1 day), 72h (3 days), 120h (5 days), and 168h (7 days) after the last injection, and the tissues was tested by LC-MS/MS to evaluate the amount of residues of florfenicol and its marker florfenicol amine, and the drug withdrawal period was calculated by WT1.4 software. The results showed that the drug withdrawal periods for florfenicol injection in chicken muscle, liver, kidney and sebum were 3.41d, 2.06d, 2.25d and 1.47d, respectively. In order to ensure the safety of veterinary drugs, consumer health and food safety, it is recommended that the withdrawal period for florfenicol injection in chicken body is 4d.