玉屏风多糖对小鼠肠黏膜免疫应答和免疫损伤的调控作用

Regulatory effects of Yupingfeng Polysaccharides on Intestinal Mucosal Immune Response and Immune Injury in Mice

关键词:  玉屏风多糖  肠黏膜免疫  细胞因子  SIgA  黏膜相关淋巴组织

Key words:  Yupingfeng polysaccharides  intestinal mucosal immunity  cytokines  Secretory IgA  gut-associated lymphoid tissue

摘要:

为研究玉屏风多糖对肠黏膜免疫应答和免疫损伤的调控作用,本试验以黄芪多糖为对照药物,以正常小鼠和免疫抑制小鼠为动物模型,150只SPF小鼠随机分为空白对照组、环磷酰胺免疫抑制对照组、玉屏风多糖治疗组、玉屏风多糖阳性对照组和黄芪多糖对照组,每组30只。多糖剂量为200mg/Kg/d,连续灌服7d,环磷酰胺剂量为80mg/Kg,隔日腹腔注射。检测SIgA、IL-2、TGF-β1和IL-6以及小肠黏膜相关淋巴组织的变化。结果发现,玉屏风多糖和黄芪多糖均可显著提高小鼠SIgA、IL-2、TGF-β1和IL-6水平(P<0.05或P<0.01),小鼠肠上皮细胞增生,杯状细胞增多,肠绒毛长度、宽度和隐窝深度均显著增加(P<0.05或P<0.01),肠上皮细胞间淋巴细胞和黏膜固有层淋巴细胞数量明显增多(P<0.05或P<0.01),PP结体积增大,淋巴细胞增生。与黄芪多糖比较,玉屏风多糖对肠黏膜免疫应答的正向调节作用更为明显。对免疫抑制小鼠,玉屏风多糖可有效拮抗环磷酰胺诱导的SIgA降低(P<0.05),并可通过调节TGF-β1(P<0.05)和IL-6(P<0.01)水平,调控Th1/Th2 漂移状态,缓解环磷酰胺所致的免疫损伤,对免疫抑制小鼠小肠黏膜相关淋巴组织也有明显的保护和恢复作用。结果表明,玉屏风多糖可从多方面调控小鼠肠黏膜的免疫应答,可有效增强肠黏膜免疫功能,缓解和改善免疫抑制小鼠肠黏膜免疫屏障的损伤。

Abstract:

To investigate and verify the regulatory effect of Yupingfeng polysaccharides (YPF-P) on intestinal mucosal immune response and immune injury, Astragalus polysaccharides (APS) performed as contrast medicine, normal mice and immunosuppressed mice were used as animal models. 150 mice at SPF grade were randomly divided into 5 groups with 30 mice in each group: blank control group, cyclophosphamide negative control group, YPF-P treatment group, YPF-P positive control group and APS control group. Polysaccharides were gavaged for 7d at 200mg/kg/d dosages, cyclophosphamide was intraperitoneal injected every other day at 80mg/Kg dosages. Then the changes of SIgA, IL-2, TGF-β1, IL-6 and gut-associated lymphoid tissue were researched. The results showed that Yupingfeng polysaccharides and Astragalus polysaccharides enhanced SIgA, IL-2, TGF-β1 and IL-6 levels significantly (P<0.05 or P<0.01), intestinal epithelial cell hyperplasia, goblet cell increase, the length and width of the intestinal villus and the depth of the crypt increased significantly (P<0.05 or P<0.01), intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) increased significantly (P<0.05 or P<0.01), Peyre"s Patch volume enlargement and lymphocytes hyperplasia. Compare with Astragalus polysaccharides, Yupingfeng polysaccharide has a more obvious positive regulatory effect on intestinal mucosal immune response. To the immunosuppressed mice, Yupingfeng polysaccharide effectively antagonized reduction of SIgA induced by cyclophosphamide (P<0.05), adjusted the levels of TGF-β1(P<0.05)and IL-6(P<0.01)to rebalance Th1/Th2 drift, alleviated the immune injury caused by cyclophosphamide, and has obvious protection and recovery effect to gut-associated lymphoid tissue. The results indicate that Yupingfeng polysaccharide can regulate mice intestinal mucosal immune response from different aspects, enhance intestinal mucosal immune function, alleviate and improve the damage of intestinal mucosal immune barrier in immunosuppression mice.