HPLC法同时测定磺胺二甲嘧啶含量和泰乐菌素组分

Simultaneous Determination of Sulfadimidine and Tylosin Compositions by HPLC

关键词:  高效液相色谱法  酒石酸泰乐菌素  磺胺二甲嘧啶

Key words:  HPLC  Tylosin Tartrate  Sulfadimidine

摘要:

本文建立了用高效液相色谱法同时测定磺胺二甲嘧啶和泰乐菌素组分含量，采用 C18（4.6×250 mm,5 μm）色谱柱；以2 mol/L高氯酸钠溶液-乙腈为流动相进行梯度洗脱；流速为1.0 mL/min；280 nm为检测波长。结果磺胺二甲嘧啶在10~200 μg/mL范围内具有良好的线性，相关系数为0.9997，检出限为0.05 μg/mL，定量限为0.15 μg/mL，低、中、高3个浓度加标的平均回收率为99.7%~100.1%，RSD在0.3%~0.5%,，泰乐菌素D峰与A峰的分离度≥3.2；泰乐菌素C峰与磺胺二甲嘧啶分离度≥30，该方法操作简便、准确、专属性强。

Abstract:

In this paper, a HPLC method for simultaneous determination of the sulfadimidine and tylosin content was developed. The sample was analyzed on a C18 column（4.6 mm×250 mm,5 μm）using 2 mol/L of sodium perchlorate-acetonitrile solution as the mobile phase for gradient elution (detection wavelength: 280 nm). The flow rate was 1.0 mL/min. The results showed good linearity (R2=0.9997) for detecting sulfadimidine in the concentration range of 10~200μg/mL. The limit of detection was 0.05μg/mL and the limit of quantitation was 0.15 μg/mL. Three spiked concentrations were used in the measurement with average recovery of?99.7%~100.1% and relative standard deviation (RSD) of?0.3%-0.5%. The separation between the D peak and A peak of tylosin was greater than 3.2 and the separation between sulfadimidine and the C peak of tylosin was greater than 30. According to the results, this HPLC method is simple and accurate with high selectivity.