高效液相色谱法测定板蓝根颗粒中腺苷的含量

Determination of Adenosine in Banlangen Granules by HPLC

关键词:  腺苷  板蓝根颗粒  高效液相色谱法  质量控制

Key words:  Adenosine  Banlangen Granules  HPLC  quality control

摘要:

建立了兽用板蓝根颗粒中腺苷含量测定的高效液相色谱法。以Symmetry C₁₈柱为固定相,甲醇-0.05mol/L磷酸二氢钾溶液(10：90)为流动相,检测波长260nm，流速为1.2ml/min，柱温30℃。采用外标法定量计算含量。腺苷的线性范围为4～20μg/ml，回归方程y=71169x-6732，R2=0.9999；平均回收率96.62%，RSD为 1.08%。方法操作简单、精密度高、稳定性好，测定结果准确可靠，可用于板蓝根颗粒中腺苷的质量控制。

Abstract:

A method was developed to determine the content of Adenosine in Banlangen Granules by HPLC, with Symmetry C₁₈ column as stationary phase, mobile phase was methyl alcohol-0.05 mol/L potassium dihydrogen phosphate (10:90),detection wavelength was 260 nm, flow rate was 1.2 mL/min, and column temperature was 30 ℃. It was quantified by external standard method and adenosine in sample quantity is 4~20 μg/ml range, show a good linear relationship, y=71169x-6732(R₂=0.9999). The recovery was 96.62%, RSD was 1.08%. This method is simple and accurate with good stability, it is suitable for determining the content of Adenosine in Banlangen Granules.