猪瘟抗体胶体金快速定量检测卡的研制

Development of Colloidal Gold Rapid and Quantitative Test Card for Classical Swine Fever Antibody

关键词:  猪瘟抗体  胶体金  检测卡

Key words:  classical swine fever antibody  colloidal gold  test card

摘要:

本研究应用胶体金免疫层析技术，结合胶体金定量读数仪研制出一种快速定量检测猪血清中猪瘟抗体水平的检测卡。该检测卡以胶体金标记高灵敏的猪瘟重组E2蛋白，同时在NC膜上包被同一蛋白，经正交实验确定最适条件，同时通过与对照线的颜色对比，应用胶体金定量读数仪，可对样本中猪瘟抗体水平进行定量。该检测卡检测方法简便、快速、稳定性好。与猪圆环病毒病、猪伪狂犬病、猪繁殖与呼吸综合征等常见猪疫病抗体均无交叉反应。检测71份猪血清样本结果与ELISA结果的符合率达90.1%。结果表明，本实验研制的猪瘟抗体胶体金快速定量检测卡可用于基层兽医站和养殖企业的检测。

Abstract:

Colloidal gold immunochromatography was applied to develop a rapid quantitative test card at the antibody level of classical swine fever(CSF) combined with colloidal gold quantitative reading meter. The test card was labeled by colloidal gold that contained high sensitive recombination E2 protein of classical swine fever, and the NC membrane was coated by recombination E2 protein. The orthogonal experiments was used to determine the optimum condition which compared with the color of control line, and then the CSF antibody level of classical swine fever can be quantified by colloidal gold quantitative reading meter. This test card is simple, fast and stable, also it has no cross-reaction with common swine disease antibodies such as porcine circovirus disease, swine pseudo rabies, porcine reproductive and respiratory syndrome. 71 serum samples of swine were detected by this research, and the coincidence rate of CSF antibody was 90.1% compared to ELISA. The results showed that the developed rapid quantitative test card of CSF antibody colloidal gold could be used in the detection of veterinary station and aquaculture enterprises.