盐酸土霉素可溶性粉在肉鸡组织中的残留消除研究

Study on Residue Elimination of Oxytetracycline Hydrochloride Soluble Powder in Broiler Tissues

关键词:  盐酸土霉素  残留消除  肉鸡  休药期

Key words:  oxytetracycline hydrochloride  residue elimination  broilers  withdrawal period

摘要:

研究在临床常规养殖环境中，对不同日龄肉鸡分别给予250mg/L和333mg/L 50%盐酸土霉素可溶性粉，集中饮水给药，每日1次，连续5d。停药后4h，1d，3d，5d，6d，7d，8d，9d，10d宰杀肉鸡，采集可食性组织（肌肉、肝脏和鸡胗）样品，肌肉、肝脏和鸡胗组织中的残留量采用UPLC-MS/MS测定。结果表明：土霉素主要残留在肝脏组织中；肌肉、肝脏和鸡胗组织的消除速率相似；停药5d后，两个剂量组的肌肉、肝脏中土霉素残留量均低于最高残留限量(MRL)。根据休药期计算软件WTl.4结果显示，在上述方法给药后，在上述两种方法给药后，建议休药期分别为7d、8d。

Abstract:

Two groups broilers of different ages were administered 250mg.L-1 and 333mg.L-1 50% oxytetracycline hydrochloride soluble powder respectively by concentrated drinking water one time every day for 5 days。The broilers were slaughtered at 4 hour,1,3,5,6,7,8,9,10 days after the last administration. Samples of edible tissues (muscle, liver and chicken gizzards) were collected.The residues of oxytetracycline were determined by UPLC-MS/MS.Results of this study showed that oxytetracycline mainly remains in liver tissue.The elimination rate of the residue was similar in muscle and liver.After 5 days of withdrawal, the residues of oxytetracycline in muscle and liver of the two dose groups were lower than Maximum Residue Limit(MRL). According to the WTl.4 calculation result, it is suggested that the period of withdrawal are 7d and 8d after the above method is given.