常山口服液抗鸡柔嫩艾美耳球虫广东分离株的疗效研究

Research on Curative Efficacy of Radix Dichroa Oral Liquid on Coccidiosis Induced by Guangdong Isolates of E.tenella in Chickens

关键词:  常山口服液  鸡球虫病  柔嫩艾美耳球虫  抗球虫药

Key words:  Radix dichroa oral Liquid (RDOL)  chicken coccidiosis  E.tenella  coccidiostat

摘要:

为了明确常山口服液治疗鸡球虫病的疗效和最佳给药剂量,本试验选取90只14日龄健康雏鸡,随机分为9组,每组10只,即常山口服液组(分别为2.5、5.0、15.0、25.0、35.0、50.0 mL/L水剂量)、妥曲珠利组(1 mL/L水)、攻虫不给药组(红对照组)和不攻虫不给药组(白对照组)。除白对照组外,每只鸡人工感染接种7×104个柔嫩艾美耳球虫广东分离株的孢子化卵囊,饮水给药7天,观察常山口服液的抗球虫疗效。结果显示,与红对照组比较,常山口服液各剂量组试验鸡只,其盲肠和十二指肠的肿胀现象明显减轻,血液性内容物明显减少,抗球虫指数(ACI)分别为87.7、136.2、141.8、136.2、109.7和108.0,均高于红对照组；常山口服液按5.0、15.0及25.0 mL/L水剂量给药,抗球虫指数都高于对照药物妥曲珠利组(ACI=124.8)。结果表明,人工感染条件下,常山口服液较化学药物妥曲珠利具有更好的抗球虫疗效,且作为中药提取物,具有低毒、低残留的优势,疗效对比试验效果理想,有望成为新型抗球虫药物。

Abstract:

To determine the curative effect and the better drug delivery dose of Radix dichroa oral liquid(RDOL)on chicken coccidiosis, 90 Lingnan-yellow chicken were divided into 9 groups randomly with 10 chickens in each group, which including RDOL group (2.5、5.0、15.0、25.0、35.0、50.0 mL/L water, respectively), toltrazuil group (1.0 mL/L water), E.tenella infected control group and healthy control group. Every chicken was inoculated 7×104 E.tenella oocysts (Guangdong isolates) on 14-day with the exception of the chicken in healthy control group, by administration of drugs through drinking water for 7 days, and the curative effects were observed. Compared with infected control group, the results showed that the cecum and duodenum in RDOL treatments were significantly alleviated, and the blood contents were reduced significantly, the anticoccidial index (ACI) of RDOL treatments were 87.7、136.2、141.8、136.2、109.7 and 108.0, respectively, which were higher than infected control groups. The anticoccidial index of 5.0, 15.0 and 25.0 mL/L water of RDOL group were higher than toltrazuil group (ACI=124.8). It is showed that RDOL has better anticocidial efficacy rather than toltrazuil under the condition of artificial infection, and the results suggested that RDOL as a kind of herbal extract is expected to become new anticoccidial drugs due to its good anticocidial efficacy, natural from source, low toxicity and residue.