枫蓼醇提液槲皮素含量测定及药效

Detection quercetin and efficacy text of Fengliao alcohol extract

关键词:  枫蓼  槲皮素  醇提液  镇痛  抗炎

摘要:

建立一种枫蓼醇提液中槲皮素含量测定方法，通过不同的实验方法和动物模型评价枫蓼醇提液的药效学，为临床合理用药提供依据。以高效液相色谱法建立枫蓼药材中槲皮素含量测定方法。采用热板法和醋酸扭体实验，观察其镇痛效果；采用二甲苯诱导小鼠耳肿胀法及醋酸致小鼠腹腔毛细血管通透性影响实验，观察其抗炎效果。槲皮素y=43215x-146476，R2=0.9995，在10～50 μg/mL浓度范围内，线性关系良好。与生理盐水组比较：热板法：醇提液高剂量组15min痛阈值显著延长（P＜0.05）；醋酸扭体法：醇提液高、中剂量组能显著降低小鼠的扭体次数（P＜0.05）；二甲苯致耳肿胀法：醇提液高剂量组肿胀抑制率显著增加（P＜0.05）；醋酸致小鼠腹腔毛细血管通透性影响：醇提液高、中剂量组吸光度显著性下降（P＜0.05），能有效降低早期炎性组织液的渗出。槲皮素检测方法准确度高，稳定性强。枫蓼醇提取液具有抗炎镇痛作用。

Abstract:

A method was established for determination of quercetin contents in Fengliao alcohol extract. Various animal models were used to evaluate the pharmacodynamics of Fengliao alcohol extract. HPLC was used to determine the content of quercetin in FengLiao alcohol extract. The analgesic effect was observed by the acetic acid induced writhing action model and hot plate induced licking paws of mouse, the anti-inflammatory effect was observed by dimethylbenzene induced ear edema model and acetic acid induced penetration increase of mouse abdominal blood capillary model The standard curve regression equation is y=43215x-14647, R2=0.9995,and the content of quercetin had good linearity with the area of peak over the range of 10～50 μg/mL . Compared with normal saline group, in the hot plate test: the threshold of 15min pain was significantly prolonged in the high dose group (P < 0.05); In the acetic acid writhing test,in high and middle dose groups of alcohol extract writhing times were reduced significantly (P<0.05); In test of ear swelling induced by xylene, the swelling inhibition rate of alcohol in high dose group was significantly increased (P<0.05); In the test of acetic acid on the permeability of capillary in the small intestine of rats,the absorbance of the high and middle dose groups decreased significantly (P<0.05). The HPLC method was simple, accurate and showed good reproducibility. The alcohol extract of Fengliao has anti-inflammatory and analgesic effects.