鸡白痢沙门氏菌阳性血清国家标准品制备用菌株的筛选

Selection of strains for preparation of the national Salmonella pullorumstandard positive sera

关键词:  鸡白痢  沙门氏菌  阳性血清  国家标准品  制备用菌株

摘要:

为了制备鸡白痢沙门氏菌阳性血清标准型国家标准品及鸡白痢沙门氏菌阳性血清变异型国家标准品,对中国兽医微生物菌种保藏管理中心保藏的10株来源背景清晰的鸡白痢沙门氏菌菌株的形态、生化特性、培养特性、血清学特性、抗原性进行了鉴定,并进行了变异检查及沙门氏菌基于invA基因的PCR检测。结果表明, CVCC79201株及CVCC79207株分别符合鸡白痢沙门氏菌标准型菌株及变异型菌株的特性,CVCC79201株菌制备的抗原与WHO标准实验室提供的Anti-salmonella Pullorum Serum (Standard Form S)及Anti-salmonella Pullorum Serum (Variant Form V)国际标准品的强阳性血清和弱阳性血清均能发生100%凝集,CVCC79207株菌制备的抗原与Anti-salmonella Pullorum Serum (Standard Form S)国际标准品的弱阳性血清发生75%凝集,与Anti-salmonella Pullorum Serum (Standard Form S)国际标准品的强阳性血清及Anti-salmonella Pullorum Serum (Variant Form V)国际标准品的强阳性血清和弱阳性血清均能发生100%凝集。用其制备免疫抗原免疫家兔后获得的阳性血清效价较高于鸡白痢沙门氏菌阳性血清国际标准品。结果证明,CVCC79201株及CVCC79207株可分别用于制备鸡白痢沙门氏菌阳性血清标准型国家标准品及鸡白痢沙门氏菌阳性血清变异型国家标准品。

Abstract:

To prepare Salmonella pullorum national standard positive sera (standard form) and Salmonella pullorum national standard positive sera (variant form), 10 strains of clear source background Salmonella pullorum were available from China veterinary culture collection center. Their bacterial morphology, biochemical characteristics, cultural characteristics, serological characteristics and antigenicity were identified. The variability check and Salmonella invA PCR detection were conducted. The results showed strain CVCC79201 and CVCC79207 were chosen for ideal strain characteristics. Antigen extracted from Strain CVCC79201 could 100% agglutinate strong/weak Anti-salmonella Pullorum Serum (Standard Form S) or strong/weak Anti-salmonella Pullorum Serum (Variant Form V), both available from the WHO standard laboratory. Antigen extracted from Strain CVCC79207 could 75% agglutinate weak Anti-salmonella Pullorum Serum (Standard Form S), and 100% agglutinate strong Anti-salmonella Pullorum Serum (Standard Form S) or strong/weak Anti-salmonella Pullorum Serum (Variant Form V). The antigen extracted from strain CVCC79201 and CVCC79207 were used to immune rabbit with the antiserum prepared. The antiserum titers were higher than the international standard positive sera. And the results declared strain CVCC79201 and CVCC79207 were used for standard and variant form Salmonella pullorum national standard positive sera preparation.