不同比例螺旋藻多糖与银杏叶提取物对小鼠抗疲劳作用的研究

Anti-fatigue effect of different proportions of Spirulina Polysaccharide and Ginkgo biloba on mice

关键词:  螺旋藻多糖(PSP)  银杏叶提取物(GBE)  抗疲劳

摘要:

[目的]研究不同比例螺旋藻多糖与银杏叶提取物复合后的抗疲劳作用。[方法] 将螺旋藻多糖(PSP)与银杏叶提取物(GBE)按不同比例复合,以200mg.kg_-1.d_-1剂量分别灌服小鼠,给药14d后造小鼠疲劳模型。通过检测小鼠力竭游泳时间、血清乳酸、血清尿素氮含量、肝脏肌糖原和肝糖原含量及心脏、肝脏、脾脏和肾脏指数变化,观察复合螺旋藻多糖的抗疲劳效果。[结果] 供试药物均可显著延长小鼠的力竭游泳时间,减少运动过程中乳酸和血清尿素氮的产生、加速乳酸的消除,还可增加肌糖原和肝糖原储备。另外,灌服供试药物对小鼠的体重增长、肝脏指数、肾脏指数无显著影响(P＞0.05),但可显著提高小鼠的心脏指数和脾脏指数(P<0.01)。[结论] 本试验的供试药物均可显著提高小鼠的运动耐力,延缓疲劳的产生或加速疲劳的消除,具有一定的抗疲劳作用。PSP与GBE以1:1和2:1比例复合使用对提高小鼠的抗疲劳能力能产生较好的协同增效作用。

Abstract:

The aim of this experiment is to study the anti-fatigue effect of different proportions of Spirulina Polysaccharide and Ginkgo biloba. Polysaccharides from spirulina platensis(PSP)and Ginkgo Biloba extract (GBE) were mixed in different proportions. The compound was treated with intragastric administration of 200mg.kg-1.d-1 for 14 days, and the mice were treated with fatigue. The exhaustive swimming time, serum lactic acid, serum urea nitrogen content, liver glycogen content and liver glycogen content were detected, heart, liver, spleen, and kidney index changes were examined in this research. Lactic acid and serum urea nitrogen were reduced during exercise. The exhaustive swimming time of mice can be significantly prolonged by the tested drugs, elimination of lactate metabolism was accelerated, and muscle glycogen and glycogen storage were increased by tested drugs. In addition, there was no significant effect on the body weight, liver and kidney index (P > 0.05), but the cardiac index and spleen index were significantly improved (P<0.01). Studies have shown that all the tested drugs have certain anti fatigue effects, which can significantly improve exercise endurance, delay fatigue or accelerate the elimination of fatigue. The combination of PSP and GBE with 1:1 and 2:1 ratio is the best synergistic effect to improve the anti-fatigue ability of mice.