猪内服和静脉给予多西环素的药代动力学研究

Pharmacokinetics of Doxycycline in swine after Oral and Intravenous Administration

关键词:  多西环素饮水剂  超高效液相色谱-串联质谱  药代动力学  猪

Key words:  Doxycycline  Drinking Water, UPLC-MS/MS, Pharmacokinetics, Swine

摘要:

建立了超高效液相色谱-串联质谱法测定猪血浆中多西环素的浓度,探讨了猪内服和静脉给予多西环素水溶液后的药代动力学特征。结果表明：猪单次内服10 mg.kg_-1体重多西环素饮水剂型水溶液后,MRT_last为12.82±3.34 h,T_1/2为11.65±4.80 h,Tmax为4.13±1.89 h,Cmax为0.87±0.48 μg.mL_-1,AUC_last为11.07±4.19μg.h.mL_-1。猪静注10 mg.kg_-1体重多西环素水溶液后,AUC_last为33.34±8.03μg.h.mL_-1,T_1/2为5.68±1.32 h,MRT_last为7.98±0.56 h。多西环素饮水剂水溶液的绝对生物利用度为33.2%。猪内服多西环素饮水剂后,具有体内吸收速度较快,达峰时间短,消除较慢,血中平均滞留时间较长等特点。

Abstract:

An UPLC-MS/MS method was established for the detection of concentration of doxycycline in porcine plasma. The pharmacokinetis of oral and intravenous doxycycline were investigated in pig. The results demonstrated that: At a single oral dose of 10 mg ? kg-1 body weight of doxycycline drinking water, MRTlast = 12.82±3.34 h, T1/2 = 11.65±4.80 h, Tmax = 4.13±