HPLC法测定喹乙醇预混剂中喹乙醇的含量

Determination of Olaquindox in Olaquindox Premix by HPLC

关键词:  喹乙醇预混剂  高效液相色谱法  测定

Key words:  olaquindox premix  high performance liquid chromatography (HPLC)  determination

摘要:

建立并验证了喹乙醇预混剂中喹乙醇含量的高效液相色谱测定法。试验采用十八烷基硅烷键合硅胶色谱柱 ( 250 mm × 4.6 mm，5 μm) ，以0.02 mol/L磷酸二氢钾溶液（用三乙胺调节pH值为6.0）-乙腈（92:8，V:V）为流动相，流速为1.0 mL/min，柱温为30 ℃，检测波长为262 nm。试验结果表明喹乙醇在5~100 μg/mL范围内线性良好，R2=1.0000，平均回收率100.2%（n=6），RSD为0.2%。本方法简便、准确、可靠，可更好地控制喹乙醇预混剂的质量。

Abstract:

The method for determination of olaquindox in olaquindox premix by HPLC was developed and validated. Analysis was performed on a C18 column ( 250 mm × 4.6 mm，5 μm) with a mode of 0.02 mol/L monopotassium phosphateaqueous solution(pH value was adjusted to 6.0 by triethylamine ) -acetonitrile(92:8) at a flow rate of 1.0 mL/min and a column temperature of 30 ℃. The determination wave length was 262 nm. The results showed that a good linear relationship was accepted in the range of 5~100 μg/mL(R2=1.0000), and the average recovery was 100.2%（n=6）with RSD% of 0.2%. This method was convenient, accurate, reliable and was suitable to control the quality of olaquindox premix.