分段补料法生产猪多杀性巴氏杆菌病活疫苗(EO630株)的试验研究

Production of Swine Pasteurella multocida Vaccine(Live,Strain EO630)by section feeding method

关键词:  猪多杀性巴氏杆菌EO630株，传统发酵工艺，分段补料发酵工艺，活菌数，生长曲线，冻干存活率

Key words:  Pasteurella multocida EO630 strain,traditional fermentation process,sub-fed fermentation process,living bacteria count,growth curve,survival rate after freeze-dry

摘要:

为了改进猪多杀性巴氏杆菌病（亦称猪肺疫）活疫苗抗原制备工艺，提高生产效率和产品质量，本试验使用分段补料发酵工艺制备猪多杀性巴氏杆菌病活疫苗（EO630株）抗原，培养第12~13小时，EO630株的活菌数达到高峰期，最高活菌数可达1.31×1010CFU/ml。按分段补料法共生产抗原5批，培养12小时后收获抗原，平均活菌数为1.17×1010CFU/ml，进行配苗和冻干疫苗5批，冻干后的平均存活率为69%。进行安全和效力检验，均合格，达到预期效果。与传统方法相比，该方法培养的活菌数有大幅度的增长，优势明显。

Abstract:

In order to improve the Swine Pasteurella Multocida (also called pig lung disease) Vaccine（Live，Strain EO630），antigen preparation technology，and to improve production efficiency and product quality，improve production efficiency and product quality，this experiment using segmented fed batch fermentation technology to produce to improve the Swine Pasteurella Multocida living vaccine （strain EO630） antigen，the living bacteria count reaches peak time at the 12-13 hours after incubation，and the highest living bacteria count is 1.31 × 1010CFU/ml．producing 5 batches of antigen by segmented Fed-batch fermentation，obtain antigen after 12 hours incubation. The average living bacteria count is 1.17 × 1010CFU/ml．Formulate the vaccine and freeze-dry 5 batch of vaccine．The average survival rate is 69% after freeze-dry．Safety test and potency test were qualified to achieve the desired results．All the results are qualified with anticipated effect．Compared with the traditional method，the living bacteria count by this method substantially increased with obvious advantages．