HPLC-ELSD法测定五加芪粉中黄芪甲苷的含量

Determination of Astragaloside in Wujiaqi powder by HPLC-ELSD

关键词:  五加芪粉  黄芪甲苷  HPLC-ELSD

Key words:  Wujiaqi powder  Astragaloside  HPLC-ELSD

摘要:

为建立五加芪粉中黄芪甲苷的含量测定方法,以控制五加芪粉的质量。采用高效液相色谱-蒸发光散射检测(HPLC-ELSD)法。色谱柱为Kromasil 100-5C18(150mm×4.6 mm, 5μm),流动相为乙腈-水35:65,流速为1.0 mL/min,柱温为30℃；采用ELSD检测器,漂移管温度为60℃,喷雾器温度为36℃,氮气压力为25psi。结果显示黄芪甲苷在1.524μg~10.16μg(r=0.9991)之间的对数值与峰面积的对数值呈良好线性关系,平均加样回收率为99.4%,RSD=4.6%(n=6)。本方法重复性良好,能准确测定五加芪粉中黄芪甲苷的含量,可对五加芪粉的质量进行控制。

Abstract:

To establish the method for determining of Astragaloside in Wujiaqi powder by HPLC-ELSD, C18 column(Kromasil 100-5C18, 150×4.6 mm, 5μm)was used with the mobile phase consisted of acetonitrile-water (35:65), the flow rate was 1.0 mL/min, column temperature was 30℃, Evaporative light scattering detection condition: drift tube temperature was 60℃, spray tube temperature was 36℃, the nitrogen pressure was 25psi. The calibration curve was linear at a range of 1.524~10.16 μg for Astragaloside (r=0.9991), the average recovery was 99.4% and RSD was 4.6%(n=6). The method was accurate with a good reproducibility and can be used as a quantitative analysis of Astragloside in Wujiaqi powder.