青海省海北州藏羊群中牛病毒性腹泻病毒和羊边界病毒的流行病学调查

Epidemiological investigation of bovine viral diarrhea virus and sheep boundary virus in Tibetan sheep in Haibei area, Qinghai Province

关键词:  藏羊  牛病毒性腹泻病毒  羊边界病毒  RT-PCR方法  流行病学调查

Key words:  Tibetan sheep  bovine viral diarrhea virus  sheep border virus  RT-PCR method  epidemiological survey.

摘要:

为掌握青海省海北州藏羊群中牛病毒性腹泻病毒和羊边界病毒的感染情况,本研究采用RT-PCR方法分别对青海省海北州的161份健康藏羊血清样品和34份腹泻藏羊组织样品进行了BVDV和BDV的抗原核酸检测。结果显示：195份样品中BVDV和BDV总阳性率分别为29.74 %和14.36 %；161份健康藏羊血清样品中BVDV和BDV平均阳性率分别为26.71 %和11.80 %,BVDV/BDV混合感染率为4.35 %；34份腹泻藏羊组织样品中BVDV和BDV平均阳性率分别为44.12 %和26.47 %,BVDV/BDV混合感染率为17.65 %。本研究表明青海省海北州健康藏羊群和腹泻藏羊群中均存在BVDV、BDV的单独感染以及混合感染,且感染情况在个别养殖场(户)较为严重,本研究为青海藏羊的综合防控措施提供了指导依据,丰富了我国羊群中BVDV和BDV的流行病学资料。

Abstract:

To master the situation of bovine viral diarrhea virus and sheep border virus infection in Tibetan sheep in Haibei area of Qinghai Province, this research uses the RT-PCR method to detect 161 healthy Tibetan sheep serum samples and 34 diarrhea Tibetan sheep tissue samples for antigen nucleic acid detection of BVDV and BDV in Haibei area of Qinghai province. The results showed that 195 copies of BVDV and BDV in the sample of total positive rates were 29.74 % and 14.36 %; 161 healthy Tibetan sheep serum samples BVDV and BDV average positive rates were 26.71 % and 11.80 %, BVDV/BDV mixed infection rate was 4.35 %; 34 diarrhea Tibet sheep BVDV and BDV tissue samples average positive rate was 44.12 % and 26.47 %, BVDV/BDV mixed infection rate was 17.65 %. This study shows that BVDV and BDV infection alone or mixed infection in health area and Tibetan diarrhea sheep in Haibei area of Qinghai Province, and infection in individual farms (households) is more serious, the study provides guidance for comprehensive prevention and control measures in Qinghai Tibetan sheep, which enrich the epidemiologic information of BVDV and BDV in China sheep.