共免疫对跳蚤叮咬引起的猫过敏性皮炎保护效果评价

Evaluation of protective effect on flea allergy dermatitis in cats by co-immunization of DNA and protein

关键词:  共免疫  跳蚤过敏性皮炎  跳蚤唾液抗原

Key words:  co-immunization  flea allergy dermatitis  flea salivary antigen 1

摘要:

共免疫重组跳蚤唾液抗原(rFSA1)和质粒pVAX-FSA1可以对跳蚤叮咬引起的猫过敏性皮炎起到良好的治疗效果。本研究评价了共免疫rFSA1和pVAX-FSA1免疫对于猫跳蚤过敏性皮炎(FAD)的预防免疫保护效果。用人工合成的跳蚤唾液抗原(FSA1)基因,分别构建原核表达质粒pET28a-FSA1和真核表达质粒pVAX1-FSA1。pET28a-FSA1转化E.coli BL21(DE3)感受态细胞,诱导表达纯化FSA1,获得rFSA1。12月龄以上猫共免疫rFSA1+pVAX-FSA1,14天进行2免后,进行跳蚤叮咬,连续4轮,跳蚤叮咬结束后,统计皮炎评分。结果显示第4轮攻跳蚤后共免疫rFSA1+pVAX-FSA1组的猫皮炎评分与FAD对照组相比极显著降低(P<0.01)。说明共免疫rFSA1+pVAX-FSA1能抑制FAD症状,有显著保护效果。rFSA1和质粒 pVAX-FSA1的共免疫可以作为预防猫跳蚤过敏性皮炎的良好办法。

Abstract:

It had a good therapeutic effect on flea allergic dermatitis (FAD) in cats by co-immunization of recombinant flea salivary antigen 1 (rFSA1) and plasmid pVAX-FSA1. This study evaluated the protective effect of immunoprophylaxis on flea allergy dermatitis in cats by co-immunization rFSA1 and pVAX-FSA1. The prokaryotic expression plasmids pET28a-FSA1 and eukaryotic expression plasmids pVAX1-FSA1 were constructed, respectively. PET28a-FSA1 was transformed into E. coli BL21 (DE3) competent cells to induce expression and purification of FSA1 to obtain rFSA1. More than 12 months old cats was immunized with rFSA1 + pVAX-FSA1, and received a second immunization after 2 weeks. After immunized, cats were challenged by fleas and sum of cat dermatitis scores were accounted. The results showed that the co-immunization group dermatitis scores were very significantly lower than FAD control group(P<0.01) at 4th flea challenge, which was slightly higher than native group. Indicating that co-immunization of rFSA1 + pVAX-FSA1 can inhibit the symptoms of FAD, have a significant protective effect. It can be used as a good way of anti-flea allergic diseases.