响应面法优化纤维素酶辅助提取淫羊藿多糖工艺

Optimization of Epimedium Polysaccharide by Response Surface Methodology

关键词:  淫羊藿多糖  酶辅助法  响应面法

Key words:  Epidmedium  Polysaccharide  enzyme assistant method  Response Surface Methodology  optimization

摘要:

利用纤维素酶辅助热水浸提法从淫羊藿中提取淫羊藿多糖，在单因素试验结果的基础上，选择酶浓度、酶提温度、酶提时间为自变量，采用三因素三水平的Box-Behnken响应面设计，优化淫羊藿多糖的提取工艺。试验结果表明，淫羊藿多糖酶辅助提取的最佳工艺参数：酶浓度110U/uL，酶提温度60℃，酶提时间100min，且酶提温度对多糖提取率的影响最大。在最佳优化条件下淫羊藿多糖提取率为10.06%，接近模型的最大预测值（10.199%）。试验为淫羊藿多糖的临床开发应用提供了依据。

Abstract:

Using enzyme assisted method from Epimedium leaves crushed(60mesh) in the extract of Epimedium Polysaccharide,on the basis of single factor test results,selection of enzyme concentration(X₁),enzyme extraction temperature(X₂),enzyme extraction time(X₃) as independent variables,using three factors and three levels of the Box-Behnken response surface design,extraction optimization of process parameters of Epimedium Polysaccharide.The result shows that the optimum process for the extraction of Epimedium Polysaccharide Enzyme were:enzyme concentration 110U/uL,enzyme extraction temperature of 60℃,enzyme extraction time 10min,and the enzyme extraction temperature had the greatest effect on the extraction rate of polysaccharide.Under the optimum conditions,the extraction rate of polysaccharide was 10.06%,which was close to the maximum value(10.199%)of the model.The results showed that this method can be used for extraction of Epimedium Polysaccharide.