| 摘要: |
| 以成功放大流感疫苗生产工艺至1500 L规模为目标，通过冷模实验研究了不同体积反应器条件下的混合时间，流体剪切力，体积氧传递系数和二氧化碳传递系数的区别。在1500 L反应器中进行培养时，转速为60 r/min，深层通气速率控制在22.5 L/min能够较好的满足供氧需求和提高CO2的移除效果，同时也降低了剪切力对细胞的损伤。病毒感染细胞60 h后，病毒滴度为211 HA units/25 μL，单位细胞病毒产量（Svy）为13145.05 virions/cell，2 L反应器的Svy为13298.70 virions/cell，两者仅相差1.16%。 |
| 关键词: 流感疫苗 MDCK细胞 生物反应器 冷模实验 放大 |
| DOI： |
| 投稿时间：2017-02-17修订日期：2017-08-01 |
| 基金项目: |
|
| Development of scale up for the production of influenza vaccine based on MDCK suspension cells |
|
| (State Key Laboratory of Bioreacter Engineering, East China University of Science and Technology) |
| Abstract: |
| In order to scale up the flu vaccine production to 1500 L animal cell culture bioreactor, cold form experiment was carried out in 1500 L bioreactor, including mixing time, liquid shear force, volumetric oxygen transfer coefficient and volumetric carbon dioxide transfer coefficient. When cultured in a 1500 L bioreactor, agitation speed and aeration rate was 60 r/min, and 22.5 L/min, respectively, which could meet the oxygen requirement, improve the effect of CO2 remove and reduce the shear force on cells. Virus-infected cells after 60 h in a 1500 L bioreactor, the virus titer was 211 HA units/25 μL, the specific virus yield (Svy) was 13298.70 virions/cell, which is the difference of 1.16% in 2 L bioreactor. |
| Key words: influenza vaccine MDCK cell bioreactor cold form experiment scale up |
