戊二醛-癸甲溴铵复合剂对大肠埃希菌和金黄色葡萄球菌的杀菌作用研究

Bactericidal Effect of Glutaraldehyde-Deciquam on Escherichia coli and Staphylococcus Aureus

关键词:  戊二醛-癸甲溴铵  大肠埃希菌  金黄色葡萄球菌  消毒  杀菌率

Key words:  Glutaraldehyde-deciquam  Escherichia coli  Staphylococcus aureus  Disinfectant  Sterilizing rate

摘要:

为评价戊二醛-癸甲溴铵复合剂在不同条件下对大肠埃希菌和金黄色葡萄球菌的杀菌效果，本研究通过悬液定量杀菌试验探究戊二醛-癸甲溴铵复合剂在不同环境温度（4℃、25℃、37℃）、不同作用时间（1 min，5 min，10 min）以及存在有机干扰物（3%、25%的牛血清白蛋白）等条件下对猪源大肠埃希菌和金黄色葡萄球菌的杀菌作用。结果显示，在没有有机物干扰时，浓度为167 mg/L的戊二醛-癸甲溴铵复合剂在4℃、25℃、 37℃下均可在5 min内完全杀灭2种受试菌（107CFU），说明环境温度对该复合剂的杀菌作用影响不明显。当有机物浓度为3%时，该复合剂在10 min内完全杀灭大肠埃希菌的最低浓度为250 mg/L，金黄色葡萄球菌为125 mg/L；有机物浓度为25%时，作用时间不低于10 min，完全杀灭大肠埃希菌的最低浓度为1000 mg/L，金黄色葡萄球菌为1250 mg/L。有机物含量高杀灭相同数量受试菌所需复合剂的量也需要加大、作用时间延长，说明有机干扰物对复合剂的杀菌作用影响较大。本研究结果提示，为达到较好的杀菌效果，建议养殖场在使用戊二醛-癸甲溴铵复合剂时的有效浓度不低于1250 mg/L、作用时间10 min以上时杀菌效果更好。

Abstract:

Abstract: To evaluate the bactericidal effect of glutaraldehyde-deciquam on Escherichia coli and Staphylococcus aureu in different environmental conditions. The suspension quantitative germicidal test was carried at different temperatures ( 4℃, 25℃, 37℃), disinfecting time (1min, 5min, 10min) and organic interference (3%, 25% bovine serum albumin). The results showed that 167mg/L of this disinfectant disinfect the target bacteria completely within 5min at 4℃, 25℃and 37℃without bovine serum albumin, indicating the slight influence of environmental temperature on bactericidal effect. The concentration of this disinfectant disinfect Escherichia coli and Staphylococcus aureus completely within 10 minutes were 250 mg/L and 125 mg/L with 3% bovine serum albumin, respectively. However, the concentration of this disinfectant disinfect Escherichia coli and Staphylococcus aureus completely within 10 minutes were 1000 mg/L and 1250 mg/L with 25% bovine serum albumin, respectively. It showed that higher level concentration and longer disinfecting time were needed for glutaraldehyde-deciquam completely disinfect same amounts of tested bacteria in the environmental condition with higher level organic interference. The results in present study suggests that the concentration no less than 1250 mg/L and the disinfecting time no less than 10 min for this disinfectant used in swine farm which can be conducted to obtain better bactericidal effect.