可乐定ELISA检测试剂盒的研制及其性能评价

Development and Performance Measurement of Clonidine ELISA Test kit

关键词:  可乐定,酶联免疫吸附测定,多克隆抗体,试剂盒

Key words:  Clonidine  Competitive ELISA  Polyclonal antibody  Test kit

摘要:

本研究通过制备可乐定完全抗原,免疫新西兰大白兔,得到特异性多克隆抗体,并和HRP标记的羊抗兔,构建间接ELISA检测法试剂盒,并进行性能测试评价。结果表明,吸光度百分比值与赛庚啶质量浓度的对数在0.1-8.1μg/L呈线性关系,相关系数R₂为0.977,半数抑制浓度(IC₅₀)为0.675μg/L,检测限为0.2μg/L,回收率为81% ～109%,具备良好的特异性,可用于动物尿样、血清和组织中的可乐定残留检测。

Abstract:

An indirect competitive ELISA assay with preparing specific anti-clonidine polyclonal antibody was developed for the rapid detection of clonidine in the animal urine. The results showed that the logarithm of clonidine concentration with the absorbance percentage shows a linear relationship between 0.1～8.1 μg/L, the correlation coefficient R₂ is 0.977, the half inhibitory concentration (IC₅₀) is 0.675 μg/L, the detection limit for the clonidine assay is 0.159 μg/L, the sample recovery is 81%～109%, the variation coefficient is 3.7%～9.2%. This assay exhibits good specificity with no any cross reactions to β-agonists . It could be used as rapid detection for the residual clonidine in the samples such as animal urine, serum and tissues..