高效液相色谱法测定二甲基半胱胺截短侧耳素的研究

Determination of Dimethyl Cysteaminyl Pleuromulin by HPLC

关键词:  高效液相色谱法  盐酸沃尼妙林中间体  二甲基半胱胺截短侧耳素  含量测定

Key words:  HPLC  Intermediates of Valnemulin Hydrochloride  Dimethyl Cysteaminyl Pleuromulin  Determination

摘要:

建立了以高效液相色谱法测定盐酸沃尼妙林关键中间体二甲基半胱胺截短侧耳素含量的检测方法。色谱条件为SepaxC18色谱柱(150×4.6mm,3μm)流动相为乙腈-磷酸缓冲液(pH2.5)(V/V= 40∶60), 流速为1ml/min,检测波长为205nm。 并对该检测方法进行了系统适用性、线性、精密度、重现性稳定性、检测限、定量限以及回收率的考察,结果均良好,可以用于盐酸沃尼妙林关键中间体的含量检测。

Abstract:

A HPLC method for the determination of Dimethyl Cysteaminyl Pleuromulin, a key intermediate of Valnemulin Hydrochloride, was established. The mobile phase consisted of acetonitrile-phosphate buffer (pH 2.5) (V / V= 40:60) with a flow rate of 1 ml / min and a detection wavelength of 205 nm. The chromatographic conditions were SepaxC18 (150 × 4.6 mm, 3 μm). The linearity, precision, reproducibility, detection limit, quantitation limit and recovery rate of the method were tested. The results were reproducible and could be applied to the content control of the key intermediates of Valnemulin Hydrochloride.