鸡痘鹌鹑化弱毒株种子批的建立及鉴定

The Seed Lots Establishment and Identification of Avian Pox Virus

关键词:  鸡痘鹌鹑化弱毒株  种子批  建立及鉴定  

Key words:  avian pox virus quail adapted strain  seed lots  establishment and identification

摘要:

为保证制苗所用毒种质量和稳定性,在对鸡痘鹌鹑化弱毒株病毒含量、病毒纯净性、特异性、免疫原性及安全性等研究的基础上,建立了鸡痘鹌鹑化弱毒株种子批。将鸡痘鹌鹑化弱毒F278 E1(1966年6月3日冻干)通过鹌鹑连续传至4代(F282 E1)后,再经鸡胚传至14代(F282 E14)。传代毒种的鉴定结果表明：抽检的各代次的毒种均无细菌、支原体、外源病毒污染,且病毒含量检测稳定,均≥10_6.2EID₅₀/0.2ml。将抽检的各代次的毒种制成活疫苗,免疫适龄鸡只后均能产生完全保护。因此,确定鸡痘鹌鹑化弱毒株基础种子代数为F282 E2～E6代,生产用毒种继代应不超过3代。种子批的建立,为鸡痘鹌鹑化弱毒株疫苗的生产奠定了基础。

Abstract:

In order to ensure the vaccine virus seed quality and stability, we established Avian Pox Virus Quail Adapted Strain vaccine virus seed lots, on the research basis of virus seed content, purity, specific, immunogenicity ,safety and expanding propagation generations．The Avian Pox Virus Quail Adapted Strain attenuated F278 E1 (June 3, 1966 gelsiccation) by Quail 4 passages (F282 E1), followed by chicken embryo passaged for 14 generations (F282 E14). The passaged identification results showed that the each generation of the virus had no bacteria, mycoplasma and exogenous virus pollution. The virus content was stable, no less than 106.2EID50/0.2ml. The various generations of the virus was made live vaccine, the chickens after immunization can produce complete protection. Therefore, we ultimately determined that the master seed was F282 E2～ E6 generation, the production virus following the generation should not exceed 3 generations. The establishment of the seed lot laid the foundation for Avian Pox Virus Quail Adapted Strain vaccine production.