贵州省猪源大肠杆菌16SrRNA甲基化酶基因调查与转移情况的研究

The research of 16SrRNA methylation enzyme gene to Escherichia E. Coli investigation and transfer in GuiZhou

关键词:  氨基糖苷类药物  耐药  16SrRNA甲基化酶  

Key words:  Aminoglycoside drug  Resistant  16SrRNA Methylation Enzyme  

摘要:

目的：对贵州省规模化养猪场的167株大肠杆菌进行16SrRNA甲基化酶基因的流行及转移情况研究，为氨基糖类药物在兽医临床上的合理使用提供理论参考。 方法：微量肉汤稀释法测定药物敏感性，PCR方法检测16SrRNA 甲基化酶基因的携带和转移；结果：阿米卡星、卡那霉素、链霉素、庆大霉素、新霉素的平均耐药率分别为17.5%、67.8%、80.9%、77.67%和75.33%。检测到 rmtB 8株，检测率为4.8%，未检测到armA。结论：rmtB基因可转移至沙门氏菌和大肠杆菌，转移之后的菌株对大部分抗菌药物耐药水平大幅提高。

Abstract:

Purpose: The object was 167 strains e.colis of the large-scale pig farms in Guizhou, explored the prevalence of 16SrRNA methylation enzyme gene and transfered research, to provide theoretical basis for the rational use of aminoglycosides. Method: Application of trace the broth dilution method to determine the sensitivity of drugs and polymerase chain reaction (PCR) to detect 16S-RMTase genes’ carry and transfer. Result: The average resistance rates of amikacin, kanamycin, streptomycin, gentamicin, neomycin was 17.5%、67.8%、80.9%、77.67% and 75.33% respectively. 8 strains of rmtB were found and the detection rate was 4.8%; armA was not detected. Conclusion: rmtB gene can be transferred to the salmonella and e.coli, and strain resistance level increased significantly after the transfered.