HPLC法同时测定连花柴芩可溶性粉中4种有效成分含量

Simultaneous Determination of Four Active Components in Lian-hua-chai-qin Soluble Power by HPLC

关键词:  连花柴芩可溶性粉  含量测定  HPLC

Key words:  Lian-hua-chai-qin soluble power  content determination  HPLC

摘要:

建立HPLC法同时测定连花柴芩可溶性粉中绿原酸、甘草苷、连翘酯苷A及黄芩苷的含量。采用Venusil XBP C18分析柱 (250 mm×4.6 mm，5 μm)，以乙腈-0.1%磷酸水为流动相，1.0 mL/min的流速下进行梯度洗脱，320 nm波长处对4种成分进行含量测定。结果表明，在此色谱条件下，4种组分分离度良好，分别在3.28~26.24 μg/mL、3.17~25.36 μg/mL、6.08~48.64 μg/mL及8.81~70.48 μg/mL范围内线性关系良好，加样回收率均大于96.61 %，RSD均小于1.75 %。该方法简便、快速、准确，重复性良好，可用于连花柴芩可溶性粉中绿原酸、甘草苷、连翘酯苷A及黄芩苷的同时测定。

Abstract:

A new HPLC method was firstly established for simultaneous determination of caffeotannic acid, liquiritin, forsythoside A and baicalin in Lian-hua-chai-qin soluble power. Analysis was performed on a venusil XBP C18 column (250 mm×4.6 mm, 5 μm). The mobile phase was considered of acetonitrile and 0.1% phosphoric acid solution, the flow rate set at 1.0 mL/min and the detected wavelength was 320 nm. Results showed four substances were separated effectively, caffeotannic acid, liquiritin, forsythoside A and baicalinin had good linearity over the range of 3.28~26.24 μg/mL、3.17~25.36 μg/mL、6.08~48.64 μg/mL and 8.81~70.48 μg/mL, respectively. The average recoveries were higher than 96.61% and RSD all below 1.75 %. The method is accurate, rapid and repeatable, can be used for simultaneous determination of caffeotannic acid, liquiritin, forsythoside A and baicalin in Lian-hua-chai-qin soluble power.