| 摘要: |
| 通过喹赛多（Cyadox, CYX）在大鼠体内消除规律研究，了解其对食品安全的影响，并为今后的药理学和毒理学研究提供较为详细的数据基础，研究建立了喹赛多及其两种主要代谢产物脱二氧喹赛多（BDCYX）和喹噁啉-2-羧酸（QCA）的提取和HPLC检测方法，并以大鼠作为研究载体，按推荐剂量连续混饲给药7天后，研究喹赛多及其两种代谢物在血浆、肌肉、肝脏中消除规律；一次性灌胃给药后研究喹赛多及其两种代谢物在排泄物中的消除规律特点。结果表明，CYX 和BDCYX在0～24 h 和 24～48 h时间段的粪便中可大量检出，在血浆、肌肉、肝脏和尿液中未检出；QCA 在6h 的肌肉中有少量残留，在肝脏中一直到72h 还有一定量的残留，在血浆和粪便中未发现其存在。本研究结果为今后喹赛多在体内处置研究提供了可直接借鉴的技术手段和理论基础。 |
| 关键词: 喹赛多 脱二氧喹赛多 喹噁啉-2-羧酸 消除规律 |
| DOI： |
| 投稿时间：2016-11-09修订日期：2017-01-19 |
| 基金项目:国家重点基础研究发展计划（“973”计划）项目。项目编号：2009CB118800 |
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| The Elimination of Cyadox and its Two Major Metabolites in Rats |
|
| (College of Plant Science and Technology of Hubei Engineering University,The Hubei Engineering Technology for Utilization of Botanical Functional Ingredients) |
| Abstract: |
| The elimination characteristic of cyadox (CYX) and its two major metabolites, 1,4-bisdesoxycyadox (BDCYX) and quinoxalie-2-carboxylic acid (QCA) were investigated in rats for its study on pharmacology, toxicology and food safety concerns. In this study, established a HPLC method for the determination of CYX, BDCYX and QCA, Following a five groups of Wistar rats (20 rats/group/sex) were fed with the diets containing cyadox (100 mg/kg) 7 consecutive days and another group was a single oral gavage of CYA (10 mg/kg bw). The results showed that CYX and BDCYX were non-detectable in plasma, muscle, liver and urine, while detectable at 0～24 h and 24～48 h in feces. QCA was detected in muscle for 6h, and detected to 72h in liver while no detectable in plasma and feces。These results provide comprehensive information for the food safety evaluation of CYA and will improve the understanding of the pharmacology and toxicology of CYA in animals. |
| Key words: CYX BDCYX QCA Elimination |
