肉桂醛体外抑制奶牛乳房炎主要致病菌的研究

Study on Study on Study on Cinnamaldehyde inhibit the main pathogenic bacteria of mastitis in dairy cow in vitro

关键词:  肉桂醛  乳房炎  抑菌  杀灭

Key words:  Cinnamaldehyde  Mastitis  Antibacterial  Sterilization

摘要:

本文旨在研究天然化合物肉桂醛对奶牛乳房炎主要致病菌—大肠杆菌、金黄色葡萄球菌和链球菌的抑菌效果。采用试管二倍稀释法测得肉桂醛对奶牛乳房炎主要致病菌的体外最小抑菌浓度（MIC）分别为金黄色葡萄球菌和标准金黄色葡萄球菌3.2 mmol/L和1.6 mmol/L；链球菌1.6 mmol/L；大肠杆菌和标准大肠杆菌6.4 mmol/L和3.2 mmol/L，且能完全抑制这三种菌的生长。肉桂醛的最小杀菌浓度（MBC）分别为金黄色葡萄球菌和标准金黄色葡萄球菌25.6 mmol/L和12.8 mmol/L；链球菌12.8 mmol/L；大肠杆菌和标准大肠杆菌25.6 mmol/L和12.8 mmol/L；在7 h内能有效杀灭这三种菌。因此，肉桂醛对奶牛乳房炎主要致病菌有抑菌和杀菌能力，是一种潜在的奶牛乳房炎的临床治疗药物。

Abstract:

The bacteriostatic effect of natural compounds cinnamaldehyde on the main pathogenic bacteria Escherichia coli, Staphylococcus aureus and Streptococcus of dairy cow mastitis was studied. Tube double dilution method was used to measure the minimum inhibitory concentration (MIC) of cinnamaldehyde on main pathogenic bacteria of dairy cow mastitis in vitro. The MIC of cinnamaldehyde against Staphylococcus aureus was 3.2 mmol/L and standards staphylococcus aureus was 1.6 mmol/L. The MIC of cinnamaldehyde against Streptococcus was 1.6mmol/L, E.coli was 6.4 mmol/L and standards E.coli was 3.2 mmol/L. Cinnamaldehyde can completely inhibit the growth of the three kinds of bacteria. The minimum bactericidal concentration (MBC) of cinnamaldehyde against Staphylococcus aureus and E.coli was 25.6 mmol/L. The MBC of cinnamaldehyde against standards Staphylococcus aureus, Streptococcus and standards E.coli was 12.8 mmol/L. These three kinds of bacteria were killed in 7 h by cinnamaldehyde. Therefore, cinnamaldehyde has antibacterial and bactericidal capability against main pathogenic bacteria of dairy cow mastitis. And it is a kind of potential clinical treatment drug of dairy cow mastitis.