| 摘要: |
| 鸡减蛋综合征(EDS)是由腺病毒科禽病毒属第三群具有血凝性的腺病毒引起的一种急性传染病,对其临床诊断的主要方法是血凝抑制(HI)试验。目前血凝抑制抗原系用禽腺病毒京911株(CVCC AV70)经尿囊腔接种易感鸭胚,收获感染鸭胚液,灭活制成,存在保存时间短和HA 效价不稳定等问题。本研究对该抗原的保存条件和时间,及其稳定性进行优化,以更符合临床诊断的需要。 |
| 关键词: 鸡减蛋综合征 血凝抑制抗原 禽腺病毒 |
| DOI： |
| 投稿时间：2016-10-17修订日期：2016-11-06 |
| 基金项目: |
|
| Study on Hemagglutination Inhibition Antigen Preparation of Egg Drop Syndrome |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| Egg Drop Syndrome(EDS) is an acute infectious disease that caused by hemagglutination adenovirus, which belongs to the third group of adenoviridae virus in avastrovirus. Main method for the clinical diagnosis is through hemagglutination inhibition (HI) test. Currently, HI antigen is prepared by inoculating susceptible duck embryo with CVCC AV70 via the allantoic cavity, then harvesting the infected duck embryo fluid, and inactivating. Whereas, there exist some problems such as short storage time and HI antigen titer instability. In this study, preservation conditions and time of the antigen and its stability were optimized to meet the needs of clinical diagnosis. |
| Key words: Egg Drop Syndrome HI Antigen avian adenovirus |
