| 摘要: |
| 为调查国内猪流感病毒流行和遗传演化状况，将2013年从山东某屠宰场的采集样品接种SPF鸡胚，分离到1株病毒，通过RT-PCR鉴定和全基因测序，并运用生物软件对病毒基因组关键氨基酸位点和遗传演化关系进行分析。结果显示，分离株A/Swine/Shandong/5513/2013 (H1N1)为欧亚类禽H1N1猪流感病毒，基因片段未发生重排，与中国大陆近几年分离株类似。HA蛋白受体结合位点具有结合哺乳动物气管上皮细胞特性；HA蛋白抗原位点与欧亚类禽H1N1猪流感代表株A/swine/Hong Kong/1780/2008(H1N1)仅有一处不同，Q196H；裂解位点氨基酸为PSIQSR/GL，PB2蛋白毒力关键氨基酸位点为T271和E627，分离株为典型低致病力毒株。本毒株的分离鉴定为分析中国大陆猪流感流行状况和分子特征提供了数据。 |
| 关键词: H1N1猪流感病毒 欧亚类禽 分子特征 |
| DOI： |
| 投稿时间：2016-09-12修订日期：2016-11-01 |
| 基金项目: |
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| Molecular Characteristic Analysis of an Isolate of Eurasian Avian-like H1N1 Swine Influenza Virus |
| 赵化阳 |
| (Beijing Veterinary Biologics Factory,Changping,Beijing) |
| Abstract: |
| To investigate SIV epidemic and mutant in China, we isolated one strain of SIV at one slaughter house in Shandong province in 2013, and it was identified as Eurasian avian-like H1N1 SIV (EA H1N1 SIV) by gene sequencing.Genes aligning analyzed by MEGA6.0 software shows that the isolate A/Swine/Shandong/5513/2013 (H1N1) is similar with EA H1N1 SIV epidemic in Northern China in recent years. Analysis of HA receptor sites amino acids illuminates that the isolate prefer to bind mammalian cells, not avian cells.Amino acids at the HA antigen site are same with A/swine/Hong Kong/1780/2008(H1N1), except the site 196 (Q196H).The isolate shows characteristic of low pathogenic according to the cleavage site amino acid（PSIQSR/GL）and T271 and E627 on PB2 coded protein. The information of this isolate is valuable for investigating SIV epidemiology in China. |
| Key words: H1N1 swine influenza virus Eurasian avian-like molecular characteristic |
