脱落酸对四氯化碳致小鼠急性肝损伤的保护作用

Hepatoprotective Effects of Abscisic Acid on Acute Liver Injury Induced by CCl4 in Mice

关键词:  脱落酸  四氯化碳  肝损伤  保肝作用

Key words:  abscisic acid  carbon tetrachloride  liver injury  hepatoprotective effect

摘要:

研究脱落酸(ABA)对四氯化碳所致小鼠急性肝损伤的保护作用。70只ICR小鼠随机分为空白组、空白对照组、模型组、联苯双酯组、ABA高、中、低剂量组，连续ig给药10 d，于末次给药1 h后，除空白组和空白对照组按0.01 mL/g注射橄榄油外，其余各组均按体重注射体分数10%的CCl4（CCl4用橄榄油稀释），6 h后，脊椎脱臼处死小鼠，收集血液，检测血清中ALT、AST、TC、TG、HDL-c水平，取肝脏制备肝匀浆测定GSH-Px、MDA、SOD活性，HE染色，光镜下观察肝组织病理变化。结果表明：ABA各剂量组均能明显改善因CCl4造成肝损伤引起的血脂异常，一定剂量的ABA可以降低肝损伤小鼠血清中ALT、AST水平的升高，降低肝匀浆中小鼠MDA含量，升高SOD、GSH-Px活性，减轻CCl4对肝组织的病理损伤。ABA对CCl4致小鼠急性肝损伤具有一定的保护作用，其保护机制可能与改善小鼠因CCl4致小鼠急性肝损伤引起的血脂异常，清除自由基，抑制脂质过氧化，减轻肝组织的损伤有关。

Abstract:

The aim is to study the protective effects of ABA on acute liver injury induced by CCl4 in mice. Seventy male ICR mice were randomly divided into seven groups: normal control group, vehicle control group, model control group, bife- ndate control group, three different dose of ABA groups consisting of high-dosage group, middle-dosage group and low-dosage group(80,40,20 mg/kg). Each group contained ten mice. All of the groups were administered by IG for 10 days. All groups were given CCl4 (10?l4 in olive oil, i.p.) by body weight, expect normal control group and vehicle control group given olive oil after 1 h at the last administration. After 6 hours, animals were sacrificed by bleeding and blood was collected. The levels of ala- nine aminotransferase(ALT), aspartate aminotransferase(AST), total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-c) were determined in serum and and superoxide dismutase(SOD) activities, malondialdehyde(MDA), GSH- Px contents in liver tissues were determined. The liver pathological changs were observed. The ABA can improved significantly the dyslipidemia caused by CCl4- induced acute liver injury in mice. A certain extent of ABA effectively decreased the levels of ALT and AST in serum and contents of MDA, increased SOD, glutathion peroxidase (GSH-Px) activities in liver. The pathological changes were relieved. ABA has a significantly hepatoprotective effect on CCl4-induced acute liver injury in mice. Protective effect of ABA on the liver may be related to its function of improving the dyslipidemia, scavenging free radicals and inhibiting lipid peroxidation, reduceing the pathological changes.