| 摘要: |
| 目的：采用体外细胞培养法研究中药黄芩中有效成分黄芩苷对鸡胚成纤维细胞（CEF）生长的影响；方法：应用细胞病变观察法（CPE）观察黄芩苷对CEF细胞生长的影响，并采用四甲基偶氮唑蓝（MTT）比色法测定黄芩苷对鸡胚成纤维细胞的最大安全浓度；结果：黄芩苷浓度在312.5μg/mL时为最大安全浓度，低于此浓度时黄芩苷能促进CEF的贴壁及生长，细胞生长良好，呈梭形，折光性强。结论：黄芩苷在中、低浓度能促进CEF细胞的贴壁和生长且对CEF细胞的作用都具有时效性，应用细胞病变观察法与MTT法，最终得出黄芩苷对CEF的最大安全浓度为312.5μg/mL。 |
| 关键词: 黄芩苷 CEF细胞 安全浓度 |
| DOI： |
| 投稿时间：2016-09-05修订日期：2017-01-03 |
| 基金项目:山东省自然科学基金（ZR2013CL010） |
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| Effect of Baicalin on safety concentrations and growth of chicken embryo fibroblast |
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| (Agricultural College of Liaocheng University) |
| Abstract: |
| Objective: The in vitro cell culture of Chinese herbs skullcap active ingredients baicalin on chick embryo growth of fibroblasts (CEF); Methods: Cytopathic observation (CPE) to investigate the effects of baicalin on CEF cell growth, and the use of tetrazolium blue (MTT) colorimetric assay of baicalin on chick embryo fibroblasts maximum safe concentration; results: the concentration of baicalin at 312.5μg / mL is the maximum safe concentration, below which the concentration of baicalin can promote the growth of CEF and adherent cells grew well, fusiform, strong refraction. Conclusion: Baicalin in low concentration could promote CEF cell adherence and growth and the role of CEF cells for both time-sensitive applications cytopathic observation and MTT assay, the final results of baicalin maximum safe concentration of CEF for 312.5μg / mL. |
| Key words: Baicalin CEF cells Safe concentration |
