精氨酸双糖苷对脂多糖诱导的巨噬细胞分泌炎症因子的影响

Effects of Arginyl-Fructosyl-Glucose on the Secretion of Inflammatory Cytokines in Macrophages Induced by Lipopolysaccharide

关键词:  精氨酸双糖苷  小鼠RAW264.7细胞  抗炎活性  炎症因子

Key words:  arginyl-fructosyl-glucose(AFG)  mouse RAW264.7 cells  anti-inflam- matory activity  inflammatory factors.

摘要:

为研究精氨酸双糖苷（AFG）体外的抗炎机制，以脂多糖（LPS）刺激小鼠单核巨噬细胞（RAW264.7）作为炎症模型，用精氨酸双糖苷（AFG）的低、中、高(5、10、20 mg/L)三个剂量进行干预后，MTT法测定细胞毒性作用，Griess法测定一氧化氮（NO）生成量，ELISA法检测细胞上清液中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）及前列腺素E2（PGE2）的分泌量。结果表明：AFG的三个不同剂量对RAW264.7细胞无抑制作用(P>0.05)，各浓度给药组的NO、IL-1β、IL-6、TNF-α和PGE2含量与LPS刺激模型组相比较都显著降低(P<0.01)，推想AFG的抗炎活性可能是通过抑制NO和PGE2等炎症介质释放，降低IL-1β、IL-6和TNF-α等炎症因子的含量而发挥了作用。

Abstract:

In this paper, we studied the anti-inflammatory mechanism of arginyl-fructosyl-glucose (AFG) in vitro. The inflammation model, which was established based on the mouse monocyte-macrophage cells (RAW264.7) stimulated by lipopolysaccharide (LPS), is intervened by three doses of AFG, low, medium and high (5, 10, 20 mg/L). After intervention, MTT approach was used to access cytotoxicity; Griess approach is used to determine the amount of nitric oxide(NO); and ELISA was used to evaluate the secretion of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) in cell supernatants. The results that: The three doses of AFG have no inhibition effect on RAW264.7 cells (P>0.05). In the control group, all contents of NO, IL-1β, IL-6, TNF-α and PGE2 are significant less than placebo group (LPS model) (P<0.01). The mechanism of AFG in anti-inflammatory activity may be contributed by inhibiting the release of inflammatory mediators of NO and PGE2, reducing the amount of IL-1β, IL-6, TNF-α and other inflammatory factors.