4种兽用注射液中非法添加倍他米松UPLC-PDA检查方法的建立

Establishment of Detection Methods for betamethasone in the 4 Kinds of Veterinary Injections by UPLC-PDA

关键词:  超高效液相法  柴胡注射液  维生素C注射液  盐酸林可霉素注射液  黄芪多糖注射液  倍他米松

Key words:  UPLC  Bupleurum injection  Vitamin C Injection  Lincomycin Hydrochloride Injection  Asragulus Polysacharin Injection  betamethasone

摘要:

建立柴胡注射液、维生素C注射液、盐酸林可霉素注射液、黄芪多糖注射液中非法添加倍他米松的超高效液相色谱检查方法。采用 Waters ACQUITY UPLC ?BEH C18色谱柱（2.1×50 mm,1.7 um），以0.75%三乙胺溶液（用磷酸调节pH为3.0）-甲醇-乙腈（50:45:5）为流动相；流速为0.3 mL?min-1；二极管阵列检测器，波长采集范围为190～400 nm，记录色谱图波长242 nm。柱温25 ℃，进样量10 uL。倍他米松质量浓度在20.0～180.0 ㎎?L-1内峰面积呈良好线性关系（R2=0.999 7，n=7)，在柴胡注射液、盐酸林可霉素注射液、维生素C注射液、黄芪多糖注射液中倍他米松的平均回收率分别为103.48%，RSD=0.15%（n=9)；0.78%，RSD=0.78%（n=9）；100.40%，RSD=1.68%（n=9）；99.16%，RSD=1.74%（n=9）。本方法简便、快速、准确，可用于柴胡注射液等四种兽用注射液中非法添加倍他米松的检测。

Abstract:

A method for the determination of betamethasone in bupleurum injection, lincomycin hydrochloride injection, vitamin C injection, and asragulus polysacharin injection was developed by UPLC-PDA. It was tested with the Waters ACQUITY UPLC ?BEH C18 column（2.1×50 mm,1.7 um）,using 0.75% tri ethylamine phosphatic liquor/Solution(pH=3.0) -methyl alcohol -acetonitrile- (50:45:5) as the mobile phase; The flow rate was 0.3ml/min; The detection wavelength was set at 242nm and simultaneously collected the spectrum at wavelengthof 190～400 nm. The column temperature had been remained in 25℃. The injection volume was 10uL. The linear range of betamethasone was 20.0-180.0 ㎎?L-1 (R2= 0.999 7,n=7) ; The mean recovery for betamethasone in bupleurum injection, lincomycin hydrochloride injection , Vitamin C Injection and asragulus polysacharin injection were 103.48%，RSD=0.15%（n=9)；0.78%，RSD=0.78%（n=9）；100.40%，RSD=1.68%（n=9）；99.16%，RSD=1.74%（n=9）. This method is accurate, easy and fast, and provides a reliable way for detecting betamethasone in bupleurum injection and another three kinds of veterinary injections.