黄连总生物碱对结核菌PhoPR 双组份系统基因转录水平影响的研究

Study on the Effect of Transcriptional Level on PhoPR Two-component System Genes by Coptis Chinensis Total Alkaloids

关键词:  黄连总生物碱  结核菌  PhoPR 双组份系统  转录水平

Key words:  Coptis chinensisStotal alkaloids  tuberculosis bacteria  PhoPR two-component system  transcriptional level

摘要:

为研究黄连总生物碱对结核菌PhoPR双组份系统基因转录水平的影响,对不同药物作用的菌液提取总RNA并进行反转录,根据GenBank 中H37Rv的PhoP (0757) 基因、PhoR(0758) 基因和SigA的基因序列,应用Primer6.0软件设计荧光定量引物并进行荧光定量PCR分析。结果显示：在高浓度黄连生物碱作用时,多药耐药结核菌MT-7、MB-1的PhoP和PhoR的基因的表达水平明显下降,并且黄连总生物碱与RPF联合作用时,多药耐药结核菌MT-7、MB-1的PhoP和PhoR的基因的表达水平明显下降,表明黄连生物碱是RFP抗菌增效剂。研究结果为结核菌抗菌增效作用机制奠定理论基础。

Abstract:

_{:To study on the effect of transcriptional level on PhoPR two-component system genes by Coptis chinensis total alkaloids}, _{the different concentrations ofCoptis chinensisStotal alkaloid or inScombination with RFP were applied to detect sensitivity to MT-7, MB-1 and H37Rv. Then the total RNA was extract and reverse transcription. The expression level of PhoP and PhoR genes belonging to the PhoPR two-component system which was related to drug resistance were analyzed and compared by realtime fluores-cence quantitative PCR. The results showed that the expression level of PhoP and phoR of MT-7, MB-1 was decreased significantly with the high concentration of total alkaloids from Coptidisrhizoma. The expression of level of PhoP and phoR of MT-7and MB-1 was decreased significantly by Coptis chinensisStotal alkaloidScombined with RFP. The results showed that Coptis chinensisStotal alkaloid was Antibacterial Synerists of RFP.}