液相色谱-串联质谱法检测猪肉、鸡肉组织中维吉尼霉素M1残留量的研究

Determination of Virginamycin M1Resedues in Pork and Chicken by LC-MS/MS

关键词:  猪肉  鸡肉  维吉尼霉素M1  兽药残留  液相色谱-串联质谱法

Key words:  pork  chicken  virginamycin M1  veterinary residue  LC-MS/MS

摘要:

为了建立可同时检测猪肉、鸡肉组织中维吉尼霉素M1药物残留的液相色谱-串联质谱分析方法，样品选用0.2%甲酸的乙腈/水（80∶20，V/V）溶液为提取液，经Oasis PRiME HLB固相萃取小柱净化，在液相色谱-串联质谱仪上进行检测。结果表明，维吉尼霉素M1在0.25～200 ng/mL浓度范围内呈现良好的线性关系，相关系数（R2）大于0.999。猪肉、鸡肉组织中维吉尼霉素M1的检测限为0.5 μg/kg，定量限为2.0 μg/kg。在2～200 μg/kg (2MRL)添加浓度水平上，回收率范围为80% ～100%,批内、批间RSD均小于8.0%。该方法具有简便快捷、灵敏度高、定性准确等特点。

Abstract:

A LC-MS/MS method was established for the determination of virginamycin M1 resedues in pork and chicken .The samples were extracted by 0.2%formate of acetonitrile-water solution (80∶20，V/V),then purified and concentrated on an Oasis PRiME HLB cartridge ,and then determined by LC-MS/MS.The results showed the good linearity in the rage of 0.25~200 ng/mL with correlation coefficients(R2) above 0.999,The limit of detection of the method was 0.5 μg/kg,and the limit of quantification was 2.0 μg/kg,The average recoveries at 2~200 μg/kg (2MRL) were 80%~100%,and intra-and inter-batch RSD were﹤8.0%.The method is simple,rapid,sensitive and accurate.