三重复合诱变选育林可霉素高产菌株

Triple Breeding of Lincomycin Producing Strain

关键词:  林可霉素  EMS-ARTP-UV三重复合诱变  高产突变株

Key words:  lincomycin  EMS-ARTP-UV mutation mutated  high-producing strain

摘要:

以提高林可霉素的生产水平为目的，将林可霉素产生菌LK15-012先用0.6%甲基磺酸乙酯(EMS)处理3 h，然后常压室温等离子体（ARTP-200W）照射90 s、最后紫外线（UV）照射50 s。结果表明：致死率为98.5%，正突变率为71.8%。最终获得2株高产突变株LK16-032和LK16-049，摇瓶效价比出发菌株LK15-012提高了42.50%和35.25%。将两株突变株分别通过100 L小试、10 t中试和60 t大生产进行发酵验证，发酵效价明显高于生产菌株。采用EMS-ARTP-UV三重复合诱变明显提高了林可霉素的生产水平。

Abstract:

In order to improve the level of production of lincomycin, clindamycin producing bacteria LK15-012 treated with 0.6% ethyl methyl sulfonic acid (EMS)3 h, the second with atmospheric pressure plasma (ARTP-200w) at room temperature irradiation of 90 s, finally by ultraviolet (UV) radiation 50 s. The results showed that the mortality rate was 98.5%, the positive mutation rate is 71.8%. Won 2 strains of high yield mutant strains LK16-032and LK16-049, shake flask titer than the starting strain LK15-012 increased by 42.50% and 35.25%.Will be high yield mutant strain respectively through the test in 100 L, 10 t pilot and 60 t fermentation carried out to verify the mass production, significantly higher than the production strains fermentation titer. The EMS-ARTP-UV triple compound mutagenesis lincomycin production levels are increased obviously.