不同伪狂犬病活疫苗免疫效力比较

Comparision of Immune Efficacy of Pseudorabies Vaccine from three Bio-factories

关键词:  伪狂犬病  活疫苗  免疫效力

Key words:  pseudo rabies  live vaccine  immune efficacy

摘要:

为临床科学选用疫苗，对来自三个生物制品厂的伪狂犬病活疫苗的免疫效力进行了评价。试验选取35日龄伪狂犬阴性猪20头，试验根据疫苗接种情况分为5组，分别为：免疫本公司ST细胞系增殖制备活疫苗组（A组）、免疫本公司鸡胚成纤维细胞增殖制备活疫苗组（B组）、免疫进口活疫苗组（C组）、免疫国产活疫苗组（D组）、免疫疫苗稀释液的对照组（E组），然后进行免疫监测和效力评价，观察不同疫苗组仔猪的抗体水平后攻毒保护情况。结果显示：一次免疫后各组抗伪狂犬病毒抗体水平增长缓慢，进行二次免疫后，抗体水平迅速增加并保持较高水平。攻毒后，从身体耐受性、体温变化、体重下降率以及gE抗体水平来看，二组C疫苗组和四组A疫苗组耐受性较强，较快恢复健康，体重下降率较小且gE抗体水平较高。

Abstract:

Immune Efficacy of pseudorabies vaccine from three bio-factories was evaluated to provide data for the selection of this kind of vaccine in practice. Twenty 35-day pigs with both sero-geative and pathogen negative was selected and divided into five groups, A, B, C, D, E, with the intramuscular injection of ST origin vaccine from our factory, CEF origin vaccine from our factory, one kind of imported vaccine from a famous company, one kind of vaccine from a company in China, and blank control, individually. Antibody titres and immune efficacy was monitored at two time points. The data demonstrated antibody titres for the gB fragment of pocine pseudorabies rised slowly after the first innoculation, then this certain antibody titres for gB elevated rapidly and stably after the second vaccination. Furthermore, some clinic index, including body resistaned, temperature fluctuation, weight loss, etc. and antibody levels for gE after poison attacking was comprehensively evaluated. The result showed that there were more tolerance, more recovery, higher gE antibody and less weight losing in group C and A..