氟苯尼考在红笛鲷体内的药代动力学研究

Pharmacokinetics of Florfenicol in Lutjanus sanguineus

关键词:  氟苯尼考  红笛鲷  药代动力学

Key words:  florfenicol  Lutjanus sanguineus  pharmacokinetics

摘要:

为研究氟苯尼考在红笛鲷体内的药代动力学特征，在水温（20±2）℃条件下，氟苯尼考以10 mg/kg单剂量腹注和口灌健康红笛鲷（Lutjanus sanguineus），采用HPLC-MS/MS测定组织中的药物浓度，数据用DAS3.0 软件分析。结果显示，两种给药方式下红笛鲷血浆药时数据均符合一级吸收二室模型；腹注给药后血浆、肝脏、肾脏和肌肉的峰浓度（Cmax）分别为10.62 μg/mL、8.36、22.57和4.76 μg/g，达峰时间（Tmax）分别为1.2、1.0、1.0和6.0 h，消除半衰期（t1/2β）分别为29.76、17.84、17.23和19.48 h；口灌给药后血浆、肝脏、肾脏和肌肉的Cmax分别为2.35 μg/mL、1.45 μg/g、4.06 μg/g和1.73 μg/g，Tmax分别为2.69、1.5、1.5和4.0 h，t1/2β分别为40.59、12.29、37.78和47.34 h。结果表明，腹注给药方式下氟苯尼考在红笛鲷体内的吸收快于口灌给药，在血浆和肝脏中的消除快于口灌给药，在肌肉和肾脏中的消除则慢于口灌给药。研究结果为氟苯尼考在临床上的合理应用提供了科学依据。

Abstract:

The pharmacokinetics of florfenicol in Lutjanus sanguineus was investigated after intraperitoneal injection and oral administration with a single dose of 10 mg/kg body weight at water temperature of (20±2) ℃. The concentrations of florfenicol in plasma and tissues were determined by means of liquid chromatography with MS/MS detection. The pharmacokinetics data were analyzed with pharmacokinetic program DAS 3.0. The results showed that the concentration-time data in plasma following intraperitoneal injection and oral adminstration were best described by a two-compartment open model with first-order absorption. After intraperitoneal injection of florfenicol, the Cmax in plasma, liver, kidney and muscle were 10.62 μg/mL, 8.36, 22.57, 4.76 μg/g，and the Tmax were 1.2, 1.0, 1.0, 6.0 h, and the t1/2β were 29.76, 17.84, 17.23, 19.48 h, respectively. After oral administration of florfenicol, the Cmax of in plasma, liver, kidney and muscle were 2.35 μg/mL, 1.45, 4.06, 1.73 μg/g, and the Tmax were 2.69, 1.5, 1.5, 4.0 h, and the t1/2β were 40.59, 12.29, 37.78, 47.34 h, respectively. The results indicated quicker absorption, faster elimination in plasma and liver but slower elimination in muscle and kidney by intraperitoneal injection than oral administration. The research results could provide a scientific basis for reasonable clinical applications.