PRRSV体外感染PAMs对IFN-γ mRNA转录水平的影响

The effects of PRRSV infected PAMs on transcription levels of IFN-γ in vitro

关键词:  PRRSV  PAMs  IFN-γ

Key words:  PRRSV  PAMs  IFN-γ

摘要:

为探讨猪繁殖与呼吸综合征病毒（PRRSV）体外感染猪肺泡巨噬细胞（PAMs）对IFN-γ mRNA转录水平的影响。选用PRRSV血清抗体和抗原均为阴性的4周龄健康仔猪3头，无菌分离PAMs，随机将其分为对照组、PRRSV感染组(PRRSV组）、PRRSV+PHA组和RRRSV+Dex组，分别在培养6、12、24、48和60 h收集PAMs。运用荧光定量PCR检测IFN-γ和PRRSV mRNA转录情况。结果显示，PRRSV mRNA转录量在感染PAMs后6-24 h逐渐升高，在48 h有所下调，在60 h再次升高，IFN-γ mRNA转录量在6-48 h逐渐升高，24和48 h显著高于对照组，而到60 h又恢复到对照组水平；与同时间点的PRRSV组相比，PRRSV+PHA组中PRRSV mRNA转录量有所下调，IFN-γ mRNA转录量有所上调；而RRRSV+Dex组PRRSV mRNA转录量有所上调，IFN-γmRNA转录量所下调。结果表明，IFN-γ 在一定程度上可以抑制PRRSV在PAMs中的增殖，IFN-γ的抑制可能是PRRSV导致细胞损伤的机制之一

Abstract:

Abstract: To explore the effects of porcine reproductive and respiratory syndrome virus (PRRSV) infected pulmonary alveolar macrophage (PAMs) on transcription levels of IFN-γin vitro.Three four weeks old piglet free of PRRSV was used in this experiment. The PAMs were isolated aseptically and divided into control group, PRRSV infection group (PRRSV group),PRRSV PHA group，PRRSV Dex group and cultured in vitro. The PAMs were collected at 6, 12, 24, 48 and 60 hours post infection. The mRNA transcription of IFN-γand PRRSV were analyzed by Real-Time PCR. The results shows that the mRNA levels of PRRSV in PRRSV group were gradually increased at 6～24 h and were decreased at 48 h ; the transcription of IFN-γ were gradually increased at 6～48 and significantly higher at 24 and 48 h than control group. PRRSV PHA group the mRNA levels of PRRSV were slightly lower and the IFN-γ is higher than PRRSV group; RRRSV Dex group the mRNA transcription of PRRSV were higher but the IFN-γ is lower.The results indicated IFN-γ can inhibit the proliferation of PRRSV in PAMs ，the inhibition of IFN -γ is probably one of the mechanisms that PRRSV cause cellular damage