鸡传染性支气管炎病毒M41株种毒的冻干鉴定

Lyophilization and Identification of Chicken Infectious Bronchitis Virus M41 Strain Seeds

关键词:  鸡传染性支气管炎病毒  冻干  鉴定

Key words:  chicken infectious bronchitis virus  lyophilization  identification

摘要:

为制备合格的鸡传染性支气管炎病毒M41株种毒，将M41株毒种接种SPF鸡胚进行传代培养，收获尿囊液、分装、冻干，并对冻干毒进行了无菌检验、支原体检验、剩余水分测定、真空度测定、外源病毒检验、病毒含量测定、特异性检验、致病性试验。结果表明，该冻干毒种各项检验均符合规定，可作为研究用种毒。本研究可为其他禽源病毒种毒的制备提供借鉴。

Abstract:

For the preparation of qualified chicken infectious bronchitis virus M41 strain seeds, M41 strain seeds were inoculated on SPF chicken embryo for subculture. Allantoic fluid was harvested, repackaged andlyophilizated, thensubsequent identification were conducted including the sterility test, mycoplasma test, determination of residual moisture, vacuum degree, exogenous virus test, virus content determination, specific identification and pathogenicity test. All the results show that lyophilizatedseedsmeet the demand of regulation and can be employed for research. This research provides the reference for the preparation of other typespoultry virus of seeds.