牛副流感病毒3型毒株的分离鉴定及基因组遗传变异分析

Isolation, Identification and Genomic Characterization of Bovine Parainfluenza Viruses Type 3

关键词:  牛副流感病毒  分离鉴定  基因组分析

Key words:  bovine parainfluenza virus  isolation and identification  genomic characterization

摘要:

从新疆地区某牛场出现发热、咳嗽等症状的多例病牛的肺组织中分离得到3株牛副流感病毒3型，分别命名为XJ03、XJ022、XJ023，对其中两株病毒XJ03和XJ023的基因组进行序列测定，测序结果显示，XJ03和XJ023毒株基因组全长分别为15474 bp（GenBank登录号为KU198929）和15475 bp，其核苷酸同源性为99.89%。同代表性的牛副流感病毒基因组比对发现，与我国的山东毒株SD0835同源性最高为99.3%，均属C型BPIV。在C型毒株中，与南韩12Q061分离株（C型）同源性最低为97.5%。F、N、HN、L、P、M蛋白基因氨基酸序列分析显示，与SD0835毒株相应序列对比，同源性分别为99.63%、92.15% 、99.48%、99.28%、98.50%和100%，部分氨基酸发生了新的变异。试验结果将为今后更好地开展BPIV3防控奠定基础。

Abstract:

Three strains of bovine parainfluenza viruses type 3(BPIV3) were successfully isolated from lung tissues of sick cattle with symptom of fever and cough emerged at a farm in Xinjiang region, and further identified by SYBR green RT-PCR. Their genome sequences were characterized. Three isolates of BPIV3 in total were obtained and designated as XJ03, XJ022 and XJ023 respectively. Genomic characterization of the two isolate XJ03 and XJ023 showed their whole genome lengths were 15475 bp and 15477 bp respectively, and were phylogenetically most closely related to the BPIV SD0835, with 99.3% identity at nucleotide, they all belong to BIPV3 genotype C. Among isolates of BIPV3 genotype C, the isolates XJ03 and XJ023 exhibited the lowest nucleotide similarity with South Korean 12Q061 strain (97.5%). In comparison to strains SD0835, the F, N, HN, L, P and M protein of isolate XJ03 have 99.63%, 92.15%, 99.48%, 99.28%, 98.50% and 100% amino acid sequence identity to those of SD0835, respectively, and there exist several new amino acid mutations. These results would be helpful for the diagnosis and control of BPIV3.