衍生气相色谱法测定鸡疫苗中游离甲醛的含量

Determination of Free Formaldehyde in Poultry Vaccine by Derivatization Capillary GC

关键词:  鸡疫苗  甲醛  2,4-二硝基苯肼衍生化  气相色谱法

Key words:  Poultry Vaccine  formaldehyde  2,4-dinitrophenylhydrazine derivative  GC

摘要:

建立了鸡疫苗中游离甲醛含量测定的衍生气相色谱法。用2,4-二硝基苯肼（2,4-DNPH）衍生鸡疫苗中的游离甲醛后，环己烷萃取衍生物，HP-5毛细管色谱柱分离，气相色谱分析，电子捕获检测器检测，外标法定量。游离甲醛质量浓度在0.05~5 mg/L内，与峰面积呈良好的线性关系，回归方程为Y=2781.9X+14.525(r=0.9999)。平均加样回收率为99.44%（RSD=1.87%）。本方法准确稳定，灵敏度高，重复性好，可用于鸡疫苗中游离甲醛的含量测定 。

Abstract:

A GC method for the determination of free formaldehyde in Poultry Vaccine has been developed. The samples were derivatized by 2,4-dinitrophenylhydrazine, extracted by cyclohexane, purifid with HP-5 capillary column, seperated by GC with an electron capture detector and qualified by external standard calibration curves. The free formaldehyde in the vaccine showed a good linear relationship to peak area within a concentration range of 0.05~5 mg/L, the regression equation was: Y=2781.9X 14.525(r=0.9999). The average recovery using the established method was 99.44% (RSD=1.87%). The derivatization capillary gas chromatography method for determination of free formaldehyde in Poultry Vaccine is developed, which is accurate, stable, sensitive, reproducible and might be used for determination of free formaldehyde in Poultry Vaccine.