| 摘要: |
| 建立了氯前列醇钠含量测定的高效液相色谱法。采用Agilent ZORBAX RX-SIL(4.6 mm×250 mm, 5 μm)为色谱柱,冰乙酸-无水乙醇-正己烷(1:100:900)为流动相,流速1.5 mL/min,检测波长220 nm,柱温35 ℃,进样量10 μL。在0.05~2.50 mg/mL浓度范围内线性关系良好(r=0.9999)。本方法结果准确可靠,可用于氯前列醇钠的质量控制。 |
| 关键词: 高效液相色谱法 氯前列醇钠 含量测定 |
| DOI： |
| 投稿时间：2016-05-19修订日期：2016-08-05 |
| 基金项目: |
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| Determination of Cloprostenol Sodium by HPLC |
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| (Zhejiang Province Institute of veterinary Drug and Feedstuff) |
| Abstract: |
| The HPLC method was established for the determination of Cloprostenol Sodium. A chromatographic column of Agilent ZORBAX RX-SIL(4.6 mm×250 mm, 5 μm)was used , with the mobile phase of acetic acid-anhydrous ethanol-n-hexane ( 1:100:900) at the flow rate of 1.5 mL/min, the detective wavelength of 220 nm , the column temperature of 35 ℃ and the injected volumn was 10 μL. The linear concentration was within the range of 0.05~2.50 mg/mL(r=0.9999). The method is accurate and available for quality control of Cloprostenol Sodium . |
| Key words: HPLC Cloprostenol Sodium determination |
