止痢散及其拆方对胃肠运动的影响

Experiment Research in Anti-diarrhoea effects of JiachuZhilisan and its disassemble prescription

关键词:  止痢散  拆方  肠推进  胃残留率  

Key words:  JiachuZhilisan  divided-form  intestinal propulsion  gastric emptying rate

摘要:

目的 研究家畜新兽药止痢散及其拆方中多叶勾儿茶、地榆和乌药组方对胃肠运动的影响,以期评价家畜新兽药止痢散及其拆方的抗腹泻作用。方法 采用实验动物模型观察止痢散及其多叶勾儿茶、地榆和乌药组方的抗腹泻效果,对正常或新斯的明致胃肠痉挛小鼠肠推进、胃残留率的影响,对家兔离体回肠平滑肌收缩活动的影响。结果,止痢散及多地组、多地乌组都可极显著减少小鼠腹泻次数(P<0.01)；同时可显著减少正常小鼠肠推进率和胃排空率(P<0.01)；但不能显著减少新斯的明致胃肠痉挛模型小鼠肠推进率和胃排空率(P>0.05)。止痢散及多地组、多地乌组都可显著减小家兔离体回肠平滑肌收缩张力的变化(P<0.01)。结论,止痢散的抗腹泻作用是与其抑制肠推进和胃排空,减小肠平滑肌收缩张力有关,但止痢散不能对抗痉挛性胃肠运动,止痢散的这一作用与方剂中的多叶勾儿茶、地榆和乌药关系密切,这为止痢散的组方和在临床上的进一步开发利用提供了数据参考。

Abstract:

To evaluate the anti-diarrhea action of JiachuZhilisan and its disassemble prescription，containing by three components, Berchemia polyphylla,Burnet and Lindera,the paper studies the gastroin -testinal motility effect of JiachuZhilisan and its disassemble prescription.The method of rat diarrhea by senna were carried out to evaluate the effects of JiachuZhilisan and its disassemble prescription on the times of diarrhea in healthy mice and the effects of them on gastric emptying and small bowel peristalsis in healthy mice and neostigmine-treated mice in vivo and smooth muscle of ileum of rabbits in vitro.The results showed that both JiachuZhilisan and its disassemble prescription could extremely significantly reduce the times of diarrhea (p<0.01),and all dose group could extremely significant suppress alvine pushing rate and gastric emptying rate of healthy mice (p<0.01).However, all the dose group could not significantly suppress intestinal propulsion and gastric emptying rate in neostigmine-treated mice(p>0.05).Both JiachuZhilisan and its ingredients could extremely significant reduce contractive?activities of smooth?muscle?of?ileum?in?rabbits in?vitro (p<0.01).The conclusion of the study is that JiachuZhilisan has good anti-diarrhea effect, which makes it mainly by inhibiting the propulsion of the gastrointestine and reduce contractive?activities of smooth?muscle?of?ileum, and this has a close relation to Berchemia polyphylla,Burnet and Lindera, hoping this can provide reference data for clinical therapy and its further development.