RP-HPLC双波长法测定四味穿心莲散中穿心莲内酯和脱水穿心莲内酯的含量

Determination of Andrographolide and Dehydroandrographolide in Siwei Chuanxinlian San by Dual-wavelength RP-HPLC

关键词:  四味穿心莲散  高效液相法  穿心莲内酯  脱水穿心莲内酯  

Key words:  Siwei Chuanxinlian San  HPLC  Andrographolide  Dehydroandrographolide

摘要:

建立了反向高效液相色谱法检测四味穿心莲散中穿心莲内脂和脱水穿心莲内酯含量的方法。试验采用十八烷基硅烷键合硅胶色谱柱( 4.6 mm × 250 mm，5 μm) ， 以甲醇- 0.2%磷酸溶液梯度洗脱，流速为1.0 mL/min，柱温为30℃；穿心莲内酯检测波长为226 nm，脱水穿心莲内酯检测波长为252 nm；穿心莲内酯脱水和脱水穿心莲内酯的含量测定范围分别为2.32~37.14 μg/mL（r=1.0000），2.00~31.94 μg/mL （r=1.0000）；加样平均回收率分别为 99.12%，98.84%；RSD分别 为0.60%，0.62%；该方法方便、准确、可靠，适用于测定四味穿心莲散中穿心莲内脂和脱水穿心莲内酯的含量。

Abstract:

To establish a RP- HPLC method for determination of andrographolide and dehydroandrographolide in Siwei Chuanxinlian San. Analysis was performed on a C18 column ( 250 mm × 4.6 mm，5 μm) with a gradient elution mode of methanol-0.2% phosphoric acid at a flow rate of 1.0 mL/min and a column temperature of 30℃. The determination wave length of andrographolide was 226 nm while dehydroandrographolide was 252 nm. The linear range was 2.32~37.14 μg /mL（r=1.0000） and 2.00~31.94 μg /mL ( r=1.0000), and the average recovery was 99.12% and 98.84% with RSD% of 0.60% and 0.62%, respectively. This method was convenient, accurate and reliable for the determination of andrographolide and dehydroandrographolide in Siwei Chuanxinlian San.