氨苄西林在鸡排泄物中的残留消除规律

Ampxicillin Residue Depletion in Chicken Excrement

关键词:  氨苄西林  高效液相色谱荧光检测法  残留消除  鸡排泄物

Key words:  Ampxicillin  HPLC with fluorescence detection  residue depletion  chicken excrement.

摘要:

为了研究氨苄西林(AMP)在鸡排泄物中残留消除规律,将鸡排泄物用乙腈去蛋白处理,再用饱和的二氯甲烷萃取后,在酸性条件下上清液用水杨醛沸水浴衍生化后,在激发波长354nm、发射波长445nm处用高效液相色谱荧光检测器检测。结果显示：该方法测定鸡排泄物中AMP的检测限为1.2 μg/kg(S/N≥3)、定量限为3.5 μg/kg(S/N≥10)。AMP在鸡排泄物样品中平均回收率为76.53%-80.41%,变异系数均低于9.77%。试验鸡按体重以60.0、120.0mg/kg.d剂量内服AMP,每天给药1次,连续5d。AMP在投药第1d时,鸡排泄物中AMP残留量被检测到,投药第5d时达到峰值。休药后AMP在鸡排泄物中残留量迅速降低。正常剂量组,休药第7d时,AMP在鸡排泄物中残留量低于检测限。双倍剂量组,在休药第9d时,鸡排泄物中AMP残留量低于检测限。AMP在鸡排泄物中的残留量与给药剂量呈正相关,鸡排泄物中高含量AMP残留存在较大的环境风险。

Abstract:

A study on Ampxicillin (AMP) residue depletion was conducted in chicken excrement. AMP was deproteinized from chicken excrement with acetonitrile, the analyte was extracted by saturated dichloromethane. The supernatant was reacted with salicylaldehyde under acidic and boiling conditions. Finally, AMP was determinated by high performance liquid chromatography (HPLC) with fluorescence detector. The excitation wavelength was set at 354 nm and emission wavelength was set at 445 nm. The average recovery in chicken excrement was 76.53%-80.41% with coefficients of variation (CV) lower than 9.77%. The limit of detection ( LOD ) was 1.2 ng/g(S/N≥3)and the limit of quantitation ( LOQ ) was 3.5ng/g(S/N≥10). After the chickens were orally administered successively AMP of 60.0 mg/.kg and 120.0 mg/kg of body weight one time every day for 5 days, AMP residues in chicken excrements was detected at the first day, the maximum residues of AMP in chicken excrement were at the 5th day. Normal dose group, AMP residues in chicken excrement was lower than LOD at the 7th withdrawal day. Double dose group, AMP residues in chicken excrement was lower than LOD at the 9th withdrawal day. The residues of AMP in chicken excrement were all positively correlated with AMP orally administered doses. There are environmental risks with high residues of AMP in chicken excrement.