氯丙那林在牛血和牛尿两种易获表征样本中的消除规律研究

Metabolism of Clorprenaline in Serum and Urine of Beef Cattles

关键词:  氯丙那林  牛血  牛尿  表征样本  消除

Key words:  clorprenaline  cattle serum  cattle urine  characteristic samples  metabolism

摘要:

利用液相色谱-串联质谱法对肉牛血清和尿液两种易获表征样本中氯丙那林消除规律进行了研究。选取4只雌性健康育肥期肉牛（500±25）kg，其中1只作为对照，3只作为试验组，每日将氯丙那林按1 mg/kg·bw 经基础日粮拌饲试验组肉牛一次，连续饲喂30 d后停止给药，继续饲喂基础日粮45 d。牛血清样品中于首饲后第2小时，即出现100 ng/mL左右氯丙那林，随后血药浓度逐步上升，并于持续给药20 d达到峰值。在停止给药后第3天，牛血清样品中氯丙那林残留量已低于方法检测限。牛尿样本中于首饲当天，即检出358～1456 ng/mL氯丙那林，连续给药期牛尿中氯丙那林残留量个体差异较大，在停止给药后21 d时，牛尿中仍有低浓度氯丙那林检出，在休药后45 d，牛尿中未检出氯丙那林。血清及尿液样本可较为准确地反映养殖过程氯丙那林非法使用情况，尿液样本的监测对活体肉牛无害化处理过程具有指导意义。

Abstract:

The metablism of Clorprenaline (CLO) in serum and urine, which are two kinds of characteristic samples could be easy acquired were investigated in 4 female fattening beef cattle by LC-MS/MS.Three cattles were supplied with CLO, mixed with concentrated feed, once perday at 1 mg/kg·bw level and last 30 days，then a 45 days off-drug period was followed. In serum, 100 ng/mL CLO were detected at 2 h after the single dose of exposure, then the blood concetration were increased progressively untill 20 days. But just after 3-day drug withdrawal, the levels of CLO were under detecion limit. In urine, the concentrations of CLO were between 358 ng/mL and 1456 ng/mL after the single dose of exposure. Due to various reasons, sharp fluctuation of the CLO level in urine were observed during the continuous exposure. Till 3-week drug withdrawal, low level of CLO could aslo be found, which were under detecion limit at 45th day. The serum and urine could be used to monitor the illegal using of CLO, and the monitoring of CLO in urine have directive significance to the innocent treatment of live beef cattles.