高效液相色谱法测定动物性食品中马波沙星残留量的研究

Determination of Marbofloxacin Residues in Animal Derived Food by HPLC

关键词:  高效液相色谱法  动物性食品  马波沙星  残留量

Key words:  high performance liquid chromatography  animal derived food  marbofloxacin  residues

摘要:

建立了高效液相色谱法测定猪肝脏、肌肉、肾脏、脂肪等组织中马波沙星残留量的方法。以氧氟沙星为内标物，将经过前处理的样品用高效液相色谱仪测定。经验证，马波沙星在肝脏、肌肉等组织中的浓度在0.02~10 μg/g范围内呈良好的线性关系，r≥0.999；方法检出限为0.01 μg/g，最低定量限为0.02 μg/g；在动物组织中以不同水平添加马波沙星，其回收率均在70%～120%之间，批内变异系数≤10%，批间变异系数≤15%。该残留检测方法具有灵敏度高、准确度及精密度高等优点，满足动物性食品安全检测的要求。

Abstract:

A method based on high performance liquid chromatography (HPLC) has been developed for the determination of marbofloxacin in pig liver, muscle, kidney, fat and other tissues. After pretreatment, the tissue samples were analyzed by high performance liquid chromatograph with ofloxacin as an internal standard. The verified results were that: a good linear relationship was obtained for concentration range of 0.02~10 μg/g in the liver, muscle and other tissues (r≥0.999); the detection limit of the method was 0.01 μg/g and the lowest limit of quantification was 0.02 μg/g; in animal tissues, marbofloxacin was added at different levels and the recoveries were at the range of 70%~120%, the intra-assay coefficient of variation was ≤10% and the inter-assay coefficient of variation was ≤15%. The residue detection method had the advantage of high sensitivity, accuracy and precision and it could meet the safety test requirements of animal derived food.