五加芪粉对鸡免疫新流二联灭活疫苗免疫效果的影响

Effect of Wujiaqi powder on immune efficacy of inactivated vaccine by Newcastle Disease virus and Avian influenza in Chicken

关键词:  五加芪粉  免疫增强剂  抗体滴度  IL-2

Key words:  Wujiaqi powder  Immunoenhancement  Antibody titer  Interleukin-2

摘要:

目的：研究五加芪粉对鸡免疫新流二联灭活疫苗后免疫功能的影响。方法：本试验选择体重相近的180只20日龄健康蛋鸡,分为6组,分别于免疫后第10、17、24、31和38天检测血清中IL-2含量及新城疫抗体水平,并于免疫后第38天检测免疫器官指数。结果：0.2g/L、0.5g/L的五加芪粉组与疫苗对照组相比,显著或极显著的提高了ND抗体水平(P<0.05,P<0.01)。不同剂量的五加芪粉组和紫锥菊末组与疫苗对照组相比,显著或极显著的提高了法氏囊指数、脾脏指数和胸腺指数(P<0.05,P<0.01)。免疫后第31天, 0.2g/L、0.5g/L五加芪粉组、紫锥菊末组与疫苗对照组相比,显著的提高了IL-2的水平(P<0.05),1g/L五加芪粉组与疫苗对照组相比,极显著的提高了IL-2的水平(P<0.01)。结论：五加芪粉可以增强机体的免疫力。

Abstract:

Object: To explore the effect of Wujiaqi powder on immune efficacy of inactivated vaccine by Newcastle Disease virus and Avian influenza in chicken. Method: One hundred and eighty 20-day-old chickens were divided into six groups, At 10,17,24,31 and 38 days after vaccination, each group was detected serum antibody titers and IL-2. The immune organ index was determined at 38 days after vaccination. Results: The groups of Wujiaqi Powder(0.2g/L, 0.5g/L) could significantly improve the immune efficacy of Newcastle disease (P<0.05, P<0.01), it also showed the bursa of fabricius index, spleen index and thymus index of the first four groups were significantly higher than that of corresponding vaccine control group(P<0.05, P<0.01) at 38 days after vaccination. At 31 days after vaccination, the groups of Wujiaqi Powder and Zizhuiju Powder could significantly improve the level of IL-2 than vaccine control group(P<0.05, P<0.01). Conclusion: Wujiaqi Powder could improve the immune efficacy.