猪肺炎支原体S株的分离与鉴定

Isolation and Identification of Strain S of Mycoplasma hyopneumoniae

关键词:  猪肺炎支原体  分离  检测  鉴定

Key words:  Mycoplasma hyopneumoniae  isolation  test  identification

摘要:

从典型猪肺炎支原体病变肺组织传代分离到一株支原体，经培养特性、血清学鉴定、生化鉴定、PCR检测及测序分析证明其为猪肺炎支原体，纯化后命名为S株。将S株P46基因和P97基因R1区的氨基酸序列与其他菌种的相应序列进行同源性分析，该菌株P46基因氨基酸序列与其他菌种同源高达99%以上，P97基因R1区的氨基酸重复数为11个，不同于其他菌株；免疫原性试验结果表明该菌株具有良好的免疫原性。

Abstract:

10 lesioned lung tissues from typical swine mycoplasmosis were grinded, cleaned, and subjected to isolation, and a mycoplasma strain was obtained. The mycoplasma was proved to be Mycoplasma hyopneumoniae(Mhp) by culture character, serological identification, biochemical identification, PCR test and sequencing. The purefied isolated strain was named Mhp strain S. Afterwards, Homology comparison was performed on genetic sequence and amino acid sequence of P46 and P97 R1 region with the available sequence of other strains, the results show that the amino acid sequence of P46 gene is more than 99% homology with other strains, the number of P97 R1 region amino acid repeat sequence of the Mhp strain S is 11, different from other strains. The immunogenicity test results showed that the isolation strain had good immunogenicity.